• Immunofluorescence analysis of U-2 OS cells using TYMS Rabbit polyclonal antibody (STJ11104456) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using TYMS Rabbit polyclonal antibody (STJ11104456) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using TYMS Rabbit polyclonal antibody (STJ11104456) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of TYSY in paraffin-embedded mouse spleen using TYMS Rabbit polyclonal antibody (STJ11104456) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and TYSY knockout (KO) 293T cells, using [KO Validated] TYSY Rabbit polyclonal antibody (STJ11104456) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TYMS antibody (220-313) (STJ11104456)

SKU:
STJ11104456

£129.50 - £639.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Thymidylate Synthase (220-313) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TYMS
Gene ID: 7298
Uniprot ID: TYSY_HUMAN
Immunogen Region: 220-313
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 220-313 of human TYSY (NP_001062.1).
Immunogen Sequence: GLGVPFNIASYALLTYMIAH ITGLKPGDFIHTLGDAHIYL NHIEPLKIQLQREPRPFPKL RILRKVEKIDDFKAEDFQIE GYNPHPTIKMEMAV
Function Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
Protein Name Thymidylate Synthase
Ts
Tsase
Database Links Reactome: R-HSA-499943
Reactome: R-HSA-69205
Cellular Localisation Nucleus
Cytoplasm
Mitochondrion
Mitochondrion Matrix
Mitochondrion Inner Membrane
Alternative Antibody Names Anti-Thymidylate Synthase antibody
Anti-Ts antibody
Anti-Tsase antibody
Anti-TYMS antibody
Anti-TS antibody
Anti-OK antibody
Anti-SW-cl.29 antibody

Information sourced from Uniprot.org

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