• Immunofluorescence analysis of U2OS cells using Thioredoxin 2 (Trx2/TXN2) Rabbit polyclonal antibody (STJ28865) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using Thioredoxin 2 (Trx2/TXN2) Rabbit polyclonal antibody (STJ28865) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Thioredoxin 2 (Trx2/Thioredoxin 2 (Trx2/TXN2) ) antibody (STJ28865) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TXN2 antibody (1-166) (STJ28865)

SKU:
STJ28865

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TXN2 (1-166) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TXN2
Gene ID: 25828
Uniprot ID: THIOM_HUMAN
Immunogen Region: 1-166
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human Thioredoxin 2 (Trx2/Thioredoxin 2 (Trx2/TXN2) ) (NP_036605.2).
Immunogen Sequence: MAQRLLLRRFLASVISRKPS QGQWPPLTSRALQTPQCSPG GLTVTPNPARTIYTTRISLT TFNIQDGPDFQDRVVNSETP VVVDFHAQWCGPCKILGPRL EKMVAKQHGKVVMAKVDIDD HTDLAIEYEVSAVPTVLAMK NGDVVDKFVGIKDEDQLEAF LKKLIG
Tissue Specificity Widely expressed in adult (at protein level) and fetal tissues.
Function Important for the control of mitochondrial reactive oxygen species homeostasis, apoptosis regulation and cell viability. Possesses a dithiol-reducing activity.
Protein Name Thioredoxin - Mitochondrial
Mtrx
Mt-Trx
Thioredoxin-2
Database Links Reactome: R-HSA-1614558
Reactome: R-HSA-3299685
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Thioredoxin - Mitochondrial antibody
Anti-Mtrx antibody
Anti-Mt-Trx antibody
Anti-Thioredoxin-2 antibody
Anti-TXN2 antibody
Anti-TRX2 antibody

Information sourced from Uniprot.org

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