• Immunofluorescence analysis of C6 cells using Beta II-Tubulin/Beta 2-Tubulin Rabbit monoclonal antibody (STJ11102157) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using Beta II-Tubulin/Beta 2-Tubulin Rabbit monoclonal antibody (STJ11102157) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Beta II-Tubulin/Beta 2-Tubulin Rabbit monoclonal antibody (STJ11102157) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Beta II-Tubulin/Beta 2-Tubulin Rabbit monoclonal antibody (STJ11102157) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-TUBB2A/TUBB2B antibody (346-445) [S7MR] (STJ11102157)

SKU:
STJ11102157

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TUBB2B
TUBB2A
Gene ID: 347733
7280
Uniprot ID: TBB2B_HUMAN
TBB2A_HUMAN
Immunogen Region: 346-445
Specificity: A synthetic peptide corresponding to a sequence within amino acids 346-445 of human Beta II-Tubulin/Beta 2-Tubulin (Q13885).
Immunogen Sequence: PNNVKTAVCDIPPRGLKMSA TFIGNSTAIQELFKRISEQF TAMFRRKAFLHWYTGEGMDE MEFTEAESNMNDLVSEYQQY QDATADEQGEFEEEEGEDEA
Tissue Specificity Q9BVA1 - High expression in brain.
Q13885 - High expression in brain, where it represents 30% of all beta-tubulins.
Alternative Antibody Names Anti-Tubulin Beta-2b Chain antibody
Anti-TUBB2B antibody
Anti-Tubulin Beta-2a Chain antibody
Anti-Tubulin Beta Class Iia antibody
Anti-TUBB2A antibody
Anti-TUBB2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance