Anti-TUBB antibody (345aa C-Term) (STJ140156)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant peptide derived from within residues 345 aa to the C-terminus of human TUBB var1 produced in E. coli.
General Information
| Short Description | Goat polyclonal Beta tubulin (345aa C-Term) antibody for use in WB in human, rat, mouse, canine and monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Goat |
| Reactivity | Human/Rat/Mouse/Canine/Monkey |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 2 mg/mL |
| Purification | This antibody is epitope-affinity purified from goat antiserum. |
| Dilution Range | WB 1:500-1:2000 |
| Formulation | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Gene Symbol | TUBB |
| Gene ID | 203068 |
| Uniprot ID | TBB5_HUMAN |
| Accession Number | ENSG00000196230 |
| Immunogen | Recombinant peptide derived from within residues 345 aa to the C-terminus of human TUBB var1 produced in E. coli. |
| Immunogen Region | 345aa C-Term |
| Immunogen Sequence | MKEVDEQMLNVQNKNSSYFV KEVDEQMLNVQNKNSSYFVE EVDEQMLNVQNKNSSYFVEW VDEQMLNVQNKNSSYFVEWI DEQMLNVQNKNSSYFVEWIP EQMLNVQNKNSSYFVEWIPN QMLNVQNKNSSYFVEWIPNN |
| Specificity | Detects endogenous levels of all variants (var1 52 KDa, var2 49.5 KDa, var3 27.3 KDa, var4 44.8KDa, var5 41.7 KDa, var6 41.7 KDa) of TUBB in different cell lines by Western blot. |
Additional Info
| Tissue Specificity | Ubiquitously expressed with highest levels in spleen, thymus and immature brain. |
| Post Translational Modifications | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility (Probable). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally (Probable). Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. |
| Function | Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. |
| Protein Name | Tubulin Beta ChainTubulin Beta-5 Chain |
| Database Links | Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-6798695Reactome: R-HSA-8854518Reactome: R-HSA-9679191 |
| Cellular Localisation | CytoplasmCytoskeleton |
| Alternative Antibody Names | Anti-Tubulin Beta Chain antibodyAnti-Tubulin Beta-5 Chain antibodyAnti-TUBB antibodyAnti-TUBB5 antibodyAnti-OK antibodyAnti-SW-cl.56 antibody |
Information sourced from Uniprot.org