• Western blot analysis of extracts of HT-1080 cells, using TSC1 Rabbit monoclonal antibody (STJ11102263) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-TSC1 antibody (1065-1164) [S3MR] (STJ11102263)

SKU:
STJ11102263

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Hamartin (1065-1164) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TSC1
Gene ID: 7248
Uniprot ID: TSC1_HUMAN
Immunogen Region: 1065-1164
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1065-1164 of human Hamartin (Q92574).
Immunogen Sequence: TTMGEASASIPTTVGSLPSS KSFLGMKARELFRNKSESQC DEDGMTSSLSESLKTELGKD LGVEAKIPLNLDGPHPSPPT PDSVGQLHIMDYNETHHEHS
Tissue Specificity Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells.
Post Translational Modifications Phosphorylation at Ser-505 does not affect interaction with TSC2.
Function Non-catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. The TSC-TBC complex acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling. The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1. Within the TSC-TBC complex, TSC1 stabilizes TSC2 and prevents TSC2 self-aggregation. Acts as a tumor suppressor. Involved in microtubule-mediated protein transport via its ability to regulate mTORC1 signaling. Also acts as a co-chaperone for HSP90AA1 facilitating HSP90AA1 chaperoning of protein clients such as kinases, TSC2 and glucocorticoid receptor NR3C1. Increases ATP binding to HSP90AA1 and inhibits HSP90AA1 ATPase activity. Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins. Recruits TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1.
Protein Name Hamartin
Tuberous Sclerosis 1 Protein
Database Links Reactome: R-HSA-1632852
Reactome: R-HSA-165181
Reactome: R-HSA-380972
Reactome: R-HSA-5628897
Reactome: R-HSA-8854214
Cellular Localisation Lysosome Membrane
Peripheral Membrane Protein
Cytoplasm
Cytosol
Recruited To Lysosomal Membranes In A Rheb-Dependent Process In Absence Of Nutrients
In Response To Nutrients
The Complex Dissociates From Lysosomal Membranes And Relocalizes To The Cytosol
Alternative Antibody Names Anti-Hamartin antibody
Anti-Tuberous Sclerosis 1 Protein antibody
Anti-TSC1 antibody
Anti-KIAA0243 antibody
Anti-TSC antibody

Information sourced from Uniprot.org

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