• Western blot analysis of various lysates using TRPM4 Rabbit monoclonal antibody (STJ11105667) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Negative control (NC) : HL-60. Exposuretime: 45s.

Anti-TRPM4 antibody (1097-1214) [S5667RM] (STJ11105667)

SKU:
STJ11105667

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Transient Receptor Potential Cation Channel Subfamily M Member 4 (1097-1214) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5667RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRPM4
Gene ID: 54795
Uniprot ID: TRPM4_HUMAN
Immunogen Region: 1097-1214
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1097-1214 of human TRPM4 (NP_060106.2).
Immunogen Sequence: RSPQPSSPALEHFRVYLSKE AERKLLTWESVHKENFLLAR ARDKRESDSERLKRTSQKVD LALKQLGHIREYEQRLKVLE REVQQCSRVLGWVAEALSRS ALLPPGGPPPPDLPGSKD
Tissue Specificity Widely expressed with a high expression in intestine and prostate. In brain, it is both expressed in whole cerebral arteries and isolated vascular smooth muscle cells. Prominently expressed in Purkinje fibers. Expressed at higher levels in T-helper 2 (Th2) cells as compared to T-helper 1 (Th1) cells. Expressed in keratocytes.
Post Translational Modifications Phosphorylation by PKC leads to increase the sensitivity to Ca(2+). Sumoylated. Desumoylated by SENP1.
Function Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway. Plays a role in keratinocyte differentiation.
Protein Name Transient Receptor Potential Cation Channel Subfamily M Member 4
Htrpm4
Calcium-Activated Non-Selective Cation Channel 1
Long Transient Receptor Potential Channel 4
Ltrpc-4
Ltrpc4
Melastatin-4
Database Links Reactome: R-HSA-3295583
Reactome: R-HSA-9717207
Cellular Localisation Isoform 1: Cell Membrane
Multi-Pass Membrane Protein
Endoplasmic Reticulum
Golgi Apparatus
Isoform 2: Cell Membrane
Alternative Antibody Names Anti-Transient Receptor Potential Cation Channel Subfamily M Member 4 antibody
Anti-Htrpm4 antibody
Anti-Calcium-Activated Non-Selective Cation Channel 1 antibody
Anti-Long Transient Receptor Potential Channel 4 antibody
Anti-Ltrpc-4 antibody
Anti-Ltrpc4 antibody
Anti-Melastatin-4 antibody
Anti-TRPM4 antibody
Anti-LTRPC4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance