Anti-TRPM1 antibody (1460-1540) (STJ119601)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TRPM1 (1460-1540) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TRPM1 |
| Gene ID | 4308 |
| Uniprot ID | TRPM1_HUMAN |
| Immunogen Region | 1460-1540 |
| Immunogen Sequence | SITDQQLTTEWQCQVQKITR SHSTDIPYIVSEAAVQAEHK EQFADMQDEHHVAEAIPRIP RLSLTITDRNGMENLLSVKP D |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1460-1540 of human TRPM1 (NP_002411.3). |
Additional Info
| Tissue Specificity | Expressed in the retina where it localizes to the outer plexiform layer. Specifically, it is expressed in retinal bipolar cells (BPCs) of the ON subtype. Highly expressed in benign melanocytic nevi and diffusely expressed in various in situ melanomas, but not detected in melanoma metastases. Also expressed in melanocytes and pigmented metastatic melanoma cell lines. In melanocytes expression appears to be regulated at the level of transcription and mRNA processing. |
| Function | Constitutively open nonselective divalent cation-conducting channels which mediate the influx of Ca(2+), Mg(2+), Mn(2+), Ba(2+), and Ni(2+) into the cytoplasm, leading to membrane depolarization. Impermeable to zinc ions. In addition, forms heteromultimeric ion channels with TRPM3 which are permeable for calcium and zinc ions. Plays an essential role for the depolarizing photoresponse of retinal ON bipolar cells. In the dark, tonic release of glutamate activates the G-protein coupled receptor for glutamate, GRM6, its activation induces the release of G(o) protein and the beta-gamma G protein dimer. Both subunits can interact and inactivate the TRPM1 channel. A light onset, induces decrease in glutamate release and deactivation of GRM6 leading to channel opening and membrane depolarization. May play a role in metastasis suppression. |
| Protein Name | Transient Receptor Potential Cation Channel Subfamily M Member 1Long Transient Receptor Potential Channel 1Ltrpc1Melastatin-1 |
| Database Links | Reactome: R-HSA-3295583Reactome: R-HSA-9824594 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinEndoplasmic Reticulum MembraneCell ProjectionAxonIn On-Bipolar Cells Is Intracellular And Located Predominantly In The ErAnd Not At The Plasma Membrane Or Golgi Apparatus |
| Alternative Antibody Names | Anti-Transient Receptor Potential Cation Channel Subfamily M Member 1 antibodyAnti-Long Transient Receptor Potential Channel 1 antibodyAnti-Ltrpc1 antibodyAnti-Melastatin-1 antibodyAnti-TRPM1 antibodyAnti-LTRPC1 antibodyAnti-MLSN antibodyAnti-MLSN1 antibody |
Information sourced from Uniprot.org