• Western blot analysis of extracts of various cell lines, using TRPC5 antibody (STJ112129) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of Neuro-2a cells using TRPC5 rabbit polyclonal antibody (STJ112129) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of SH-SY5Y cells using TRPC5 rabbit polyclonal antibody (STJ112129) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-TRPC5 antibody (800-900) (STJ112129)

SKU:
STJ112129

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRPC5 (800-900) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRPC5
Gene ID: 7224
Uniprot ID: TRPC5_HUMAN
Immunogen Region: 800-900
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 800-900 of human TRPC5 (NP_036603.1).
Immunogen Sequence: NLGCKKKTCHGPPLIRTMPR SSGAQGKSKAESSSKRSFMG PSLKKLGLLFSKFNGHMSEP SSEPMYTISDGIVQQHCMWQ DIRYSQMEKGKAEACSQSEI N
Tissue Specificity Expressed in brain with higher levels in fetal brain. Found in cerebellum and occipital pole.
Function Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective. May also be activated by intracellular calcium store depletion. Mediates calcium-dependent phosphatidylserine externalization and apoptosis in neurons via its association with PLSCR1.
Protein Name Short Transient Receptor Potential Channel 5
Trpc5
Transient Receptor Protein 5
Trp-5
Htrp-5
Htrp5
Database Links Reactome: R-HSA-3295583
Reactome: R-HSA-418890
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Short Transient Receptor Potential Channel 5 antibody
Anti-Trpc5 antibody
Anti-Transient Receptor Protein 5 antibody
Anti-Trp-5 antibody
Anti-Htrp-5 antibody
Anti-Htrp5 antibody
Anti-TRPC5 antibody
Anti-TRP5 antibody

Information sourced from Uniprot.org

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