Anti-TRPA1 antibody (STJ13100388)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from rat TRPA1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse.
General Information
| Short Description | Rabbit polyclonal anti-TRPA1 for use in IHC and WB in Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. Use at a concentration of 10-50 ug/ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20ยฐC for long term storage and refrigerated at 2-8ยฐC for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Trpa1 |
| Gene ID | 277328 |
| Uniprot ID | TRPA1_MOUSE |
| Immunogen | A synthetic peptide from rat TRPA1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse. |
| Specificity | Specific for TRPA1. |
Additional Info
| Post Translational Modifications | TRPA1 activation by electrophiles occurs though covalent modification of specific cysteine residues in the N-terminal cytoplasmic domain. Hydroxylation is required for TRPA1 activity inhibition in normoxia. In hypoxia, the decrease in oxygen concentration diminishes the activity of the hydroxylase EGLN1, thus relieving TRPA1 from inhibition and ultimately leading to channel activation. Oxidation of Cys-634 and Cys-859 in hyperoxia may override the hydroxylase EGLN1-mediated inhibition, causing TRPA1 activation. |
| Function | Ligand-activated Ca(2+)-permeable, nonselective cation channel. Involved in pain detection and possibly also in cold perception, oxygen concentration perception, cough, itch, and inner ear function. Has a relatively high Ca(2+) selectivity, with a preference for divalent over monovalent cations (Ca(2+) > Ba(2+) > Mg(2+) > NH4(+) > Li(+) > K(+)), the influx of cation into the cytoplasm, leads to membrane depolarization. Has a central role in the pain response to endogenous inflammatory mediators, such as bradykinin and to a diverse array of irritants. Activated by a large variety of structurally unrelated electrophilic and non-electrophilic chemical compounds, such as allylthiocyanate (AITC) from mustard oil or wasabi, cinnamaldehyde, diallyl disulfide (DADS) from garlic, and acrolein, an environmental irritant. Electrophilic ligands activate TRPA1 by interacting with critical N-terminal Cys residues in a covalent manner. Non-electrophile agonists bind at distinct sites in the transmembrane domain to promote channel activation. Also acts as an ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. |
| Protein Name | Transient Receptor Potential Cation Channel Subfamily A Member 1Ankyrin-Like With Transmembrane Domains Protein 1Wasabi Receptor |
| Database Links | Reactome: R-MMU-3295583 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Transient Receptor Potential Cation Channel Subfamily A Member 1 antibodyAnti-Ankyrin-Like With Transmembrane Domains Protein 1 antibodyAnti-Wasabi Receptor antibodyAnti-Trpa1 antibodyAnti-Anktm1 antibody |
Information sourced from Uniprot.org