Anti-TRPA1 antibody (STJ13100388)

SKU:
STJ13100388-500

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Host: NZ White Rabbit
Applications: IHC/WB
Reactivity: Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Nz White Rabbit polyclonal antibody anti-TRPA1 is suitable for use in Immunohistochemistry and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Shipped as lyophilised. Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.
Purification: Ammonium sulphate precipitation
Dilution Range: Use at a concentration of 10-50 µg/ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.
Storage Instruction: Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Gene Symbol: Trpa1
Gene ID: 277328
Uniprot ID: TRPA1_MOUSE
Specificity: Specific for TRPA1.
Immunogen: A synthetic peptide from rat TRPA1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse.
Post Translational Modifications TRPA1 activation by electrophiles occurs though covalent modification of specific cysteine residues in the N-terminal cytoplasmic domain. Hydroxylation is required for TRPA1 activity inhibition in normoxia. In hypoxia, the decrease in oxygen concentration diminishes the activity of the hydroxylase EGLN1, thus relieving TRPA1 from inhibition and ultimately leading to channel activation. Oxidation of Cys-634 and Cys-859 in hyperoxia may override the hydroxylase EGLN1-mediated inhibition, causing TRPA1 activation.
Function Receptor-activated non-selective cation channel involved in pain detection and possibly also in cold perception, oxygen concentration perception, cough, itch, and inner ear function. Shows 8-fold preference for divalent over monovalent cations. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of irritants, such as allylthiocyanate (AITC) found in mustard oil or wasabi, cinnamaldehyde, diallyl disulfide (DADS) from garlic, and acrolein, an irritant from tears gas and vehicle exhaust fumes. Acts also as an ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Is activated by a large variety of structurally unrelated electrophilic and non-electrophilic chemical compounds. Electrophilic ligands activate TRPA1 by interacting with critical N-terminal Cys residues in a covalent manner, whereas mechanisms of non-electrophilic ligands are not well determined. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system.
Protein Name Transient Receptor Potential Cation Channel Subfamily A Member 1
Ankyrin-Like With Transmembrane Domains Protein 1
Wasabi Receptor
Database Links Reactome: R-MMU-3295583
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Transient Receptor Potential Cation Channel Subfamily A Member 1 antibody
Anti-Ankyrin-Like With Transmembrane Domains Protein 1 antibody
Anti-Wasabi Receptor antibody
Anti-Trpa1 antibody
Anti-Anktm1 antibody

Information sourced from Uniprot.org

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