| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TRMT61A |
| Gene ID: | 115708 |
| Uniprot ID: | TRM61_HUMAN |
| Immunogen Region: | 1-289 |
| Immunogen Sequence: | MSFVAYEELIKEGDTAILSL GHGAMVAVRVQRGAQTQTRH GVLRHSVDLIGRPFGSKVTC GRGGWVYVLHPTPELWTLNL PHRTQILYSTDIALITMMLE LRPGSVVCESGTGSGSVSHA IIRTIAPTGHLHTVEFHQQR AEKAREEFQEHRVGRWVTVR TQDVCRSGFGVSHVADAVFL DIPSPWEAVGHAWDALKVEG GRFCSFSPCIEQVQRTCQAL AARGFSELSTLEVLPQVYN |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human TRMT61A (NP_689520.2). |
| Function | Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Catalytic subunit of mRNA N(1)-methyltransferase complex, which mediates methylation of adenosine residues at the N(1) position of a small subset of mRNAs: N(1) methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries. |
| Protein Name | TrnaAdenine(58-N(1-Methyltransferase Catalytic Subunit Trmt61aMrna Methyladenosine-N(1-Methyltransferase Catalytic Subunit Trmt61aTrna(M1a58-Methyltransferase Subunit Trmt61aTrna(M1a58mtase Subunit Trmt61a |
| Database Links | Reactome: R-HSA-6782315 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Trna antibodyAnti-Adenine(58-N(1-Methyltransferase Catalytic Subunit Trmt61a antibodyAnti-Mrna Methyladenosine-N(1-Methyltransferase Catalytic Subunit Trmt61a antibodyAnti-Trna(M1a58-Methyltransferase Subunit Trmt61a antibodyAnti-Trna(M1a58mtase Subunit Trmt61a antibodyAnti-TRMT61A antibodyAnti-C14orf172 antibodyAnti-TRM61 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and PNP knockout (KO) 293T cells, using [KO Validated] PNP Rabbit polyclonal antibody (STJ11100761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89698/358183/STJ11100761_1__08836.1713122495.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PNP knockout (KO) 293T cells, using [KO Validated] PNP Rabbit polyclonal antibody (STJ11100761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
