• Western blot analysis of various lysates, using Cip4 Rabbit monoclonal antibody (STJ11104097) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-TRIP10 antibody (1-100) [S4097RM] (STJ11104097)

SKU:
STJ11104097

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Cdc42-Interacting Protein 4 (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4097RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRIP10
Gene ID: 9322
Uniprot ID: CIP4_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cip4 (Q15642).
Immunogen Sequence: MDWGTELWDQFEVLERHTQW GLDLLDRYVKFVKERTEVEQ AYAKQLRSLVKKYLPKRPAK DDPESKFSQQQSFVQILQEV NDFAGQRELVAENLSVRVCL
Tissue Specificity Expressed in brain, colon, heart, kidney, liver, lung, megakaryocyte, ovary, pancreas, peripheral blood lymphocytes, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus and trachea.
Post Translational Modifications Tyrosine phosphorylated. Also phosphorylated by PKA.
Function Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling. Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL.
Protein Name Cdc42-Interacting Protein 4
Protein Felic
Salt Tolerant Protein
Hstp
Thyroid Receptor-Interacting Protein 10
Tr-Interacting Protein 10
Trip-10
Database Links Reactome: R-HSA-8856828
Reactome: R-HSA-9013406
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Cortex
Lysosome
Golgi Apparatus
Cell Membrane
Cell Projection
Phagocytic Cup
Translocates To The Plasma Membrane In Response To Insulin Stimulation
And This May Require Active Rhoq
Localizes To Cortical Regions Coincident With F-Actin
To Lysosomes And To Sites Of Phagocytosis In Macrophages
Also Localizes To The Golgi
And This Requires Akap9
Isoform 5: Cytoplasm
Perinuclear Region
Alternative Antibody Names Anti-Cdc42-Interacting Protein 4 antibody
Anti-Protein Felic antibody
Anti-Salt Tolerant Protein antibody
Anti-Hstp antibody
Anti-Thyroid Receptor-Interacting Protein 10 antibody
Anti-Tr-Interacting Protein 10 antibody
Anti-Trip-10 antibody
Anti-TRIP10 antibody
Anti-CIP4 antibody
Anti-STOT antibody
Anti-STP antibody

Information sourced from Uniprot.org

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