| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TRIM24 |
| Gene ID: | 8805 |
| Uniprot ID: | TIF1A_HUMAN |
| Immunogen Region: | 600-820 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 600-820 of human TRIM24 (NP_056989.2). |
| Immunogen Sequence: | DGKAFGSPMIDLSSPVGGSY NLPSLPDIDCSSTIMLDNIV RKDTNIDHGQPRPPSNRTVQ SPNSSVPSPGLAGPVTMTSV HPPIRSPSASSVGSRGSSGS SSKPAGADSTHKVPVVMLEP IRIKQENSGPPENYDFPVVI VKQESDEESRPQNANYPRSI LTSLLLNSSQSSTSEETVLR SDAPDSTGDQPGLHQDNSSN GKSEWLDPSQKSPLHVGETR K |
| Post Translational Modifications | Phosphorylated at Ser-768 by ATM kinase induces ubiquitination and degradation during DNA damage. Sumoylated. Undergoes ubiquitination-mediated degradation in response to DNA damage. |
| Function | Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. During the DNA damage response, participates in an autoregulatory feedback loop with TP53. Early in response to DNA damage, ATM kinase phosphorylates TRIM24 leading to its ubiquitination and degradation. After sufficient DNA repair has occurred, TP53 activates TRIM24 transcription, ultimately leading to TRIM24-mediated TP53 ubiquitination and degradation. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes. Participates also in innate immunity by mediating the specific 'Lys-63'-linked ubiquitination of TRAF3 leading to activation of downstream signal transduction of the type I IFN pathway. Additionally, negatively regulates NLRP3/CASP1/IL-1beta-mediated pyroptosis and cell migration probably by ubiquitinating NLRP3. |
| Protein Name | Transcription Intermediary Factor 1-Alpha Tif1-Alpha E3 Ubiquitin-Protein Ligase Trim24 Ring Finger Protein 82 Ring-Type E3 Ubiquitin Transferase Tif1-Alpha Tripartite Motif-Containing Protein 24 |
| Database Links | Reactome: R-HSA-1839117 Reactome: R-HSA-5655302 Reactome: R-HSA-6802952 |
| Cellular Localisation | Nucleus Cytoplasm Mitochondrion Colocalizes With Sites Of Active Transcription Predominantly Nuclear Translocated From Nucleus To Mitochondria To Mediate Antiviral Immunity Localizes To Sites Of Dna Damage |
| Alternative Antibody Names | Anti-Transcription Intermediary Factor 1-Alpha antibody Anti-Tif1-Alpha antibody Anti-E3 Ubiquitin-Protein Ligase Trim24 antibody Anti-Ring Finger Protein 82 antibody Anti-Ring-Type E3 Ubiquitin Transferase Tif1-Alpha antibody Anti-Tripartite Motif-Containing Protein 24 antibody Anti-TRIM24 antibody Anti-RNF82 antibody Anti-TIF1 antibody Anti-TIF1A antibody |
Information sourced from Uniprot.org