| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TRIM24 (600-820) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TRIM24 |
| Gene ID: |
8805 |
| Uniprot ID: |
TIF1A_HUMAN |
| Immunogen Region: |
600-820 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 600-820 of human TRIM24 (NP_056989.2). |
| Immunogen Sequence: |
DGKAFGSPMIDLSSPVGGSY NLPSLPDIDCSSTIMLDNIV RKDTNIDHGQPRPPSNRTVQ SPNSSVPSPGLAGPVTMTSV HPPIRSPSASSVGSRGSSGS SSKPAGADSTHKVPVVMLEP IRIKQENSGPPENYDFPVVI VKQESDEESRPQNANYPRSI LTSLLLNSSQSSTSEETVLR SDAPDSTGDQPGLHQDNSSN GKSEWLDPSQKSPLHVGETR K |
| Post Translational Modifications | Phosphorylated at Ser-768 by ATM kinase induces ubiquitination and degradation during DNA damage. Sumoylated. Undergoes ubiquitination-mediated degradation in response to DNA damage. |
| Function | Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. During the DNA damage response, participates in an autoregulatory feedback loop with TP53. Early in response to DNA damage, ATM kinase phosphorylates TRIM24 leading to its ubiquitination and degradation. After sufficient DNA repair has occurred, TP53 activates TRIM24 transcription, ultimately leading to TRIM24-mediated TP53 ubiquitination and degradation. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes. Participates also in innate immunity by mediating the specific 'Lys-63'-linked ubiquitination of TRAF3 leading to activation of downstream signal transduction of the type I IFN pathway. Additionally, negatively regulates NLRP3/CASP1/IL-1beta-mediated pyroptosis and cell migration probably by ubiquitinating NLRP3. |
| Protein Name | Transcription Intermediary Factor 1-AlphaTif1-AlphaE3 Ubiquitin-Protein Ligase Trim24Ring Finger Protein 82Ring-Type E3 Ubiquitin Transferase Tif1-AlphaTripartite Motif-Containing Protein 24 |
| Database Links | Reactome: R-HSA-1839117Reactome: R-HSA-5655302Reactome: R-HSA-6802952 |
| Cellular Localisation | NucleusCytoplasmMitochondrionColocalizes With Sites Of Active TranscriptionPredominantly NuclearTranslocated From Nucleus To Mitochondria To Mediate Antiviral ImmunityLocalizes To Sites Of Dna Damage |
| Alternative Antibody Names | Anti-Transcription Intermediary Factor 1-Alpha antibodyAnti-Tif1-Alpha antibodyAnti-E3 Ubiquitin-Protein Ligase Trim24 antibodyAnti-Ring Finger Protein 82 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Tif1-Alpha antibodyAnti-Tripartite Motif-Containing Protein 24 antibodyAnti-TRIM24 antibodyAnti-RNF82 antibodyAnti-TIF1 antibodyAnti-TIF1A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
