| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TRAF3 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TRAF3 |
| Gene ID: |
7187 |
| Uniprot ID: |
TRAF3_HUMAN |
| Immunogen Region: |
1-100 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TRAF3 (NP_003291.2). |
| Immunogen Sequence: |
MESSKKMDSPGALQTNPPLK LHTDRSAGTPVFVPEQGGYK EKFVKTVEDKYKCEKCHLVL CSPKQTECGHRFCESCMAAL LSSSSPKCTACQESIVKDKV |
| Post Translational Modifications | Undergoes 'Lys-48'-linked polyubiquitination, leading to its proteasomal degradation in response to signaling by TNFSF13B, TLR4 or through CD40. 'Lys-48'-linked polyubiquitinated form is deubiquitinated by OTUD7B, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B. Undergoes 'Lys-63'-linked ubiquitination during early stages of virus infection, and 'Lys-48'-linked ubiquitination during later stages. Undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to TLR3 and TLR4 signaling. 'Lys-63'-linked ubiquitination can be mediated by TRIM35. Deubiquitinated by OTUB1, OTUB2 and OTUD5. Undergoes 'Lys-63'-linked deubiquitination by MYSM1 to terminate the pattern-recognition receptors/PRRs pathways. Undergoes also 'Lys-29'-linked ubiquitination on Cys-56 and Cys-124 by NEDD4L.leading to increased 'Lys-48'- and 'Lys-63'-linked ubiquitination as well as increased binding to TBK1. TLR4 signals emanating from bacteria containing vesicles trigger 'Lys-33'-linked polyubiquitination that promotes the assembly of the exocyst complex thereby connecting innate immune signaling to the cellular trafficking apparatus. Deubiquitinated by USP25 during viral infection, leading to TRAF3 stabilization and type I interferon production. Ubiquitinated at Lys-329 by the SCF(FBXL2) complex, leading to its degradation by the proteasome. 'Lys-63'-linked ubiquitination by FBXO11 in a NEDD8-dependent manner promotes the amplification of IFN-I signaling. (Microbial infection) Cleaved by enterovirus D68 protease 2A.leading to inhibition of NF-kappa-B or IFN-beta triggered by TRAF3. |
| Function | Cytoplasmic E3 ubiquitin ligase that regulates various signaling pathways, such as the NF-kappa-B, mitogen-activated protein kinase (MAPK) and interferon regulatory factor (IRF) pathways, and thus controls a lot of biological processes in both immune and non-immune cell types. In TLR and RLR signaling pathways, acts as an E3 ubiquitin ligase promoting the synthesis of 'Lys-63'-linked polyubiquitin chains on several substrates such as ASC that lead to the activation of the type I interferon response or the inflammasome. Following the activation of certain TLRs such as TLR4, acts as a negative NF-kappa-B regulator, possibly to avoid unregulated inflammatory response, and its degradation via 'Lys-48'-linked polyubiquitination is required for MAPK activation and production of inflammatory cytokines. Alternatively, when TLR4 orchestrates bacterial expulsion, TRAF3 undergoes 'Lys-33'-linked polyubiquitination and subsequently binds to RALGDS, mobilizing the exocyst complex to rapidly expel intracellular bacteria back for clearance. Acts also as a constitutive negative regulator of the alternative NF-kappa-B pathway, which controls B-cell survival and lymphoid organ development. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14. |
| Protein Name | Tnf Receptor-Associated Factor 3Cd40 Receptor-Associated Factor 1Craf1Cd40-Binding ProteinCd40bpLmp1-Associated Protein 1Lap1Ring-Type E3 Ubiquitin Transferase Traf3 |
| Database Links | Reactome: R-HSA-5602571Reactome: R-HSA-5668541Reactome: R-HSA-5676594Reactome: R-HSA-5689896Reactome: R-HSA-9013973Reactome: R-HSA-918233Reactome: R-HSA-936440Reactome: R-HSA-936964Reactome: R-HSA-9692916Reactome: R-HSA-9705671 |
| Cellular Localisation | CytoplasmEndosomeMitochondrionUndergoes Endocytosis Together With Tlr4 Upon Lps SignalingCo-Localized To Mitochondria With Trim35 |
| Alternative Antibody Names | Anti-Tnf Receptor-Associated Factor 3 antibodyAnti-Cd40 Receptor-Associated Factor 1 antibodyAnti-Craf1 antibodyAnti-Cd40-Binding Protein antibodyAnti-Cd40bp antibodyAnti-Lmp1-Associated Protein 1 antibodyAnti-Lap1 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Traf3 antibodyAnti-TRAF3 antibodyAnti-CAP-1 antibodyAnti-CRAF1 antibodyAnti-TRAFAMN antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
