• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g TCTP/TPT1 antibody (STJ11101817). Western blot was performed from the immunoprecipitate using TCTP/TPT1 antibody (STJ11101817) at a dilution of 1:1000.
  • Immunohistochemistry analysis of TCTP/TPT1 in paraffin-embedded rat brain using TCTP/TPT1 Rabbit monoclonal antibody (STJ11101817) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TCTP/TPT1 Rabbit monoclonal antibody (STJ11101817) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TPT1 antibody (1-100) [S7MR] (STJ11101817)

SKU:
STJ11101817

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-TCTP (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000 IHC-P 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TPT1
Gene ID: 7178
Uniprot ID: TCTP_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TCTP/TPT1 (P13693).
Immunogen Sequence: MIIYRDLISHDEMFSDIYKI REIADGLCLEVEGKMVSRTE GNIDDSLIGGNASAEGPEGE GTESTVITGVDIVMNHHLQE TSFTKEAYKKYIKDYMKSIK
Tissue Specificity Found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC). Expressed in placenta and prostate.
Function Involved in calcium binding and microtubule stabilization. Acts as a negative regulator of TSC22D1-mediated apoptosis, via interaction with and destabilization of TSC22D1 protein.
Protein Name Translationally-Controlled Tumor Protein
Tctp
Fortilin
Histamine-Releasing Factor
Hrf
P23
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Translationally-Controlled Tumor Protein antibody
Anti-Tctp antibody
Anti-Fortilin antibody
Anti-Histamine-Releasing Factor antibody
Anti-Hrf antibody
Anti-P23 antibody
Anti-TPT1 antibody

Information sourced from Uniprot.org

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