• Immunofluorescence analysis of HeLa cells using TPM3 antibody (STJ25942). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using TPM3 antibody (STJ25942) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-TPM3 antibody (1-285) (STJ25942)

SKU:
STJ25942

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TPM3 (1-285) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TPM3
Gene ID: 7170
Uniprot ID: TPM3_HUMAN
Immunogen Region: 1-285
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human TPM3 (NP_689476.2).
Immunogen Sequence: MMEAIKKKMQMLKLDKENAL DRAEQAEAEQKQAEERSKQL EDELAAMQKKLKGTEDELDK YSEALKDAQEKLELAEKKAA DAEAEVASLNRRIQLVEEEL DRAQERLATALQKLEEAEKA ADESERGMKVIENRALKDEE KMELQEIQLKEAKHIAEEAD RKYEEVARKLVIIEGDLERT EERAELAESKCSELEEELKN VTNNLKSLEAQAEKYSQKED KYEEEIKILTDKLKEAETR
Function Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
Protein Name Tropomyosin Alpha-3 Chain
Gamma-Tropomyosin
Tropomyosin-3
Tropomyosin-5
Htm5
Database Links Reactome: R-HSA-390522
Reactome: R-HSA-445355
Reactome: R-HSA-9013424
Reactome: R-HSA-9725370
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Tropomyosin Alpha-3 Chain antibody
Anti-Gamma-Tropomyosin antibody
Anti-Tropomyosin-3 antibody
Anti-Tropomyosin-5 antibody
Anti-Htm5 antibody
Anti-TPM3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance