• Immunoprecipitation analysis of 300 Mu g extracts of Mouse skeletal muscle cells using 3 Mu g TPM2 antibody (STJ25941). Western blot was performed from the immunoprecipitate using TPM2 antibody (STJ25941) at a dilution of 1:1000.
  • Immunohistochemistry analysis of TPM2 in paraffin-embedded mouse heart using TPM2 Rabbit polyclonal antibody (STJ25941) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TPM2 in paraffin-embedded human stomach using TPM2 Rabbit polyclonal antibody (STJ25941) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TPM2 in paraffin-embedded rat heart using TPM2 Rabbit polyclonal antibody (STJ25941) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TPM2 Rabbit polyclonal antibody (STJ25941) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TPM2 antibody (1-100) (STJ25941)

SKU:
STJ25941

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TPM2 (1-100) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TPM2
Gene ID: 7169
Uniprot ID: TPM2_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TPM2 (NP_003280.2).
Immunogen Sequence: MDAIKKKMQMLKLDKENAID RAEQAEADKKQAEDRCKQLE EEQQALQKKLKGTEDEVEKY SESVKEAQEKLEQAEKKATD AEADVASLNRRIQLVEEELD
Tissue Specificity Present in primary breast cancer tissue, absent from normal breast tissue.
Post Translational Modifications Phosphorylated on Ser-61 by PIK3CG. Phosphorylation on Ser-61 is required for ADRB2 internalization.
Function Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization.
Protein Name Tropomyosin Beta Chain
Beta-Tropomyosin
Tropomyosin-2
Database Links Reactome: R-HSA-390522
Reactome: R-HSA-445355
Cellular Localisation Cytoplasm
Cytoskeleton
Associates With F-Actin Stress Fibers
Alternative Antibody Names Anti-Tropomyosin Beta Chain antibody
Anti-Beta-Tropomyosin antibody
Anti-Tropomyosin-2 antibody
Anti-TPM2 antibody
Anti-TMSB antibody

Information sourced from Uniprot.org

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