• Immunofluorescence analysis of U-2 OS cells using TPI1 Rabbit polyclonal antibody (STJ118179) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of TPI1 in paraffin-embedded mouse kidney using TPI1 Rabbit polyclonal antibody (STJ118179) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TPI1 in paraffin-embedded human lung cancer using TPI1 Rabbit polyclonal antibody (STJ118179) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TPI1 in paraffin-embedded rat kidney using TPI1 Rabbit polyclonal antibody (STJ118179) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TPI1 Rabbit polyclonal antibody (STJ118179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TPI1 antibody (1-249) (STJ118179)

SKU:
STJ118179

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TPI1 (1-249) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TPI1
Gene ID: 7167
Uniprot ID: TPIS_HUMAN
Immunogen Region: 1-249
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human TPI1 (NP_000356.1).
Immunogen Sequence: MAPSRKFFVGGNWKMNGRKQ SLGELIGTLNAAKVPADTEV VCAPPTAYIDFARQKLDPKI AVAAQNCYKVTNGAFTGEIS PGMIKDCGATWVVLGHSERR HVFGESDELIGQKVAHALAE GLGVIACIGEKLDEREAGIT EKVVFEQTKVIADNVKDWSK VVLAYEPVWAIGTGKTATPQ QAQEVHEKLRGWLKSNVSDA VAQSTRIIYGGSVTGATCKE LASQPDVDGFLVGGASLKP
Function Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis. It is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids.
Protein Name Triosephosphate Isomerase
Tim
Methylglyoxal Synthase
Triose-Phosphate Isomerase
Database Links Reactome: R-HSA-70171
Reactome: R-HSA-70263
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Triosephosphate Isomerase antibody
Anti-Tim antibody
Anti-Methylglyoxal Synthase antibody
Anti-Triose-Phosphate Isomerase antibody
Anti-TPI1 antibody
Anti-TPI antibody

Information sourced from Uniprot.org

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