| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TOP1MT (470-550) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TOP1MT |
| Gene ID: |
116447 |
| Uniprot ID: |
TOP1M_HUMAN |
| Immunogen Region: |
470-550 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 470-550 of human TOP1MT (NP_443195.1). |
| Immunogen Sequence: |
RATPSTFEKSMQNLQTKIQA KKEQVAEARAELRRARAEHK AQGDGKSRSVLEKKRRLLEK LQEQLAQLSVQATDKEENKQ V |
| Tissue Specificity | Ubiquitous.highest in skeletal muscle, heart, brain and fetal liver. |
| Function | Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. |
| Protein Name | Dna Topoisomerase I - MitochondrialTop1mt |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Dna Topoisomerase I - Mitochondrial antibodyAnti-Top1mt antibodyAnti-TOP1MT antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
