• Immunofluorescence analysis of PC-12 cells using TOMM34 Rabbit polyclonal antibody (STJ26684) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using TOMM34 Rabbit polyclonal antibody (STJ26684) at 1:800 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 45s.

Anti-TOMM34 antibody (1-309) (STJ26684)

SKU:
STJ26684

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TOMM34 (1-309) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TOMM34
Gene ID: 10953
Uniprot ID: TOM34_HUMAN
Immunogen Region: 1-309
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human TOMM34 (NP_006800.2).
Immunogen Sequence: MAPKFPDSVEELRAAGNESF RNGQYAEASALYGRALRVLQ AQGSSDPEEESVLYSNRAAC HLKDGNCRDCIKDCTSALAL VPFSIKPLLRRASAYEALEK YPMAYVDYKTVLQIDDNVTS AVEGINRMTRALMDSLGPEW RLKLPSIPLVPVSAQKRWNS LPSENHKEMAKSKSKETTAT KNRVPSAGDVEKARVLKEEG NELVKKGNHKKAIEKYSESL LCSNLESATYSNRALCYLV
Tissue Specificity Ubiquitous.
Function Plays a role in the import of cytosolically synthesized preproteins into mitochondria. Binds the mature portion of precursor proteins. Interacts with cellular components, and possesses weak ATPase activity. May be a chaperone-like protein that helps to keep newly synthesized precursors in an unfolded import compatible state.
Protein Name Mitochondrial Import Receptor Subunit Tom34
Htom34
Translocase Of Outer Membrane 34 Kda Subunit
Cellular Localisation Cytoplasm
Mitochondrion Outer Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Mitochondrial Import Receptor Subunit Tom34 antibody
Anti-Htom34 antibody
Anti-Translocase Of Outer Membrane 34 Kda Subunit antibody
Anti-TOMM34 antibody
Anti-URCC3 antibody

Information sourced from Uniprot.org

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