• Western blot analysis of lysates from wild type (WT) and TOMM34 knockout (KO) HeLa cells, using [KO Validated] TOMM34 Rabbit polyclonal antibody (STJ11100836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TOMM34 antibody (1-309) (STJ11100836)

SKU:
STJ11100836

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TOMM34 (1-309) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TOMM34
Gene ID: 10953
Uniprot ID: TOM34_HUMAN
Immunogen Region: 1-309
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human TOMM34 (NP_006800.2).
Immunogen Sequence: MAPKFPDSVEELRAAGNESF RNGQYAEASALYGRALRVLQ AQGSSDPEEESVLYSNRAAC HLKDGNCRDCIKDCTSALAL VPFSIKPLLRRASAYEALEK YPMAYVDYKTVLQIDDNVTS AVEGINRMTRALMDSLGPEW RLKLPSIPLVPVSAQKRWNS LPSENHKEMAKSKSKETTAT KNRVPSAGDVEKARVLKEEG NELVKKGNHKKAIEKYSESL LCSNLESATYSNRALCYLV
Tissue Specificity Ubiquitous.
Function Plays a role in the import of cytosolically synthesized preproteins into mitochondria. Binds the mature portion of precursor proteins. Interacts with cellular components, and possesses weak ATPase activity. May be a chaperone-like protein that helps to keep newly synthesized precursors in an unfolded import compatible state.
Protein Name Mitochondrial Import Receptor Subunit Tom34
Htom34
Translocase Of Outer Membrane 34 Kda Subunit
Cellular Localisation Cytoplasm
Mitochondrion Outer Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Mitochondrial Import Receptor Subunit Tom34 antibody
Anti-Htom34 antibody
Anti-Translocase Of Outer Membrane 34 Kda Subunit antibody
Anti-TOMM34 antibody
Anti-URCC3 antibody

Information sourced from Uniprot.org

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