• Immunofluorescence analysis of U-87MG cells using Tenascin C Rabbit polyclonal antibody (STJ11100126) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using Tenascin C Rabbit polyclonal antibody (STJ11100126) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Tenascin C Rabbit polyclonal antibody (STJ11100126) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse brain using Tenascin C Rabbit polyclonal antibody (STJ11100126) at 1:400 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:180s.

Anti-TNC antibody (1990-2201) (STJ11100126)

SKU:
STJ11100126

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TNC (1990-2201) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TNC
Gene ID: 3371
Uniprot ID: TENA_HUMAN
Immunogen Region: 1990-2201
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1990-2201 of human Tenascin C (NP_002151.2).
Immunogen Sequence: NGDTTSGLYTIYLNGDKAEA LEVFCDMTSDGGGWIVFLRR KNGRENFYQNWKAYAAGFGD RREEFWLGLDNLNKITAQGQ YELRVDLRDHGETAFAVYDK FSVGDAKTRYKLKVEGYSGT AGDSMAYHNGRSFSTFDKDT DSAITNCALSYKGAFWYRNC HRVNLMGRYGDNNHSQGVNW FHWKGHEHSIQFAEMKLRPS NFRNLEGRRKRA
Tissue Specificity Detected in fibroblasts (at protein level).
Function Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells.
Protein Name Tenascin
Tn
Cytotactin
Gmem
Gp 150-225
Glioma-Associated-Extracellular Matrix Antigen
Hexabrachion
Ji
Myotendinous Antigen
Neuronectin
Tenascin-C
Tn-C
Database Links Reactome: R-HSA-216083
Reactome: R-HSA-3000170
Reactome: R-HSA-3000178
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Tenascin antibody
Anti-Tn antibody
Anti-Cytotactin antibody
Anti-Gmem antibody
Anti-Gp 150-225 antibody
Anti-Glioma-Associated-Extracellular Matrix Antigen antibody
Anti-Hexabrachion antibody
Anti-Ji antibody
Anti-Myotendinous Antigen antibody
Anti-Neuronectin antibody
Anti-Tenascin-C antibody
Anti-Tn-C antibody
Anti-TNC antibody
Anti-HXB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance