| Host: | Goat |
| Applications: | Pep-ELISA/IF/FC |
| Reactivity: | Human/Mouse/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-TMP21/TMED10 (C-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:1000.WB: Preliminary experiments gave an approx 75kDa band in Rat Pancreas lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | TMED10 |
| Gene ID: | 10972 |
| Uniprot ID: | TMEDA_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_006818.3 |
| Immunogen Sequence: | YLRRFFKAKKLIE |
| Protein Name | Transmembrane Emp24 Domain-Containing Protein 10Protein Tmed1021 Kda Transmembrane-Trafficking ProteinS31i125S31iii125Tmp-21-ITransmembrane Protein Tmp21P23P24 Family Protein Delta-1P24delta1P24delta |
| Database Links | Reactome: R-HSA-204005Reactome: R-HSA-5694530Reactome: R-HSA-6807878Reactome: R-HSA-6811434 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type I Membrane ProteinEndoplasmic Reticulum-Golgi Intermediate Compartment MembraneGolgi Apparatus MembraneGolgi ApparatusCis-Golgi Network MembraneTrans-Golgi Network MembraneCytoplasmic VesicleSecretory Vesicle MembraneCell MembraneMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-Transmembrane Emp24 Domain-Containing Protein 10 antibodyAnti-Protein Tmed10 antibodyAnti-21 Kda Transmembrane-Trafficking Protein antibodyAnti-S31i125 antibodyAnti-S31iii125 antibodyAnti-Tmp-21-I antibodyAnti-Transmembrane Protein Tmp21 antibodyAnti-P23 antibodyAnti-P24 Family Protein Delta-1 antibodyAnti-P24delta1 antibodyAnti-P24delta antibodyAnti-TMED10 antibodyAnti-TMP21 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and TMED10 knockout (KO) 293T cells, using [KO Validated] TMED10 Rabbit polyclonal antibody (STJ11100063) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89133/357582/STJ11100063_1__14749.1713121714.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TMED10 knockout (KO) 293T cells, using [KO Validated] TMED10 Rabbit polyclonal antibody (STJ11100063) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
