• Western blot analysis of extracts of MCF-7 cells, using TMEM189 antibody (STJ110678) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of TMEM189 in paraffin-embedded human stomach using TMEM189 Rabbit polyclonal antibody (STJ110678) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver using TMEM189 antibody (STJ110678) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TMEM189 in paraffin-embedded human liver using TMEM189 Rabbit polyclonal antibody (STJ110678) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human stomach using TMEM189 antibody (STJ110678) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from MCF-7 cells, using TMEM189 Rabbit polyclonal antibody (STJ110678) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TMEM189 antibody (171-270) (STJ110678)

SKU:
STJ110678

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TMEM189 (171-270) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PEDS1
Gene ID: 387521/387522
Uniprot ID: PDES1_HUMAN
Immunogen Region: 171-270
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 171-270 of human TMEM189 (NP_954580.1).
Immunogen Sequence: FVFCLIIFGTFTNQIHKWSH TYFGLPRWVTLLQDWHVILP RKHHRIHHVSPHETYFCITT GWLNYPLEKIGFWRRLEDLI QGLTGEKPRADDMKWAQKIK
Function Plasmanylethanolamine desaturase involved in plasmalogen biogenesis in the endoplasmic reticulum membrane. Plasmalogens are glycerophospholipids with a hydrocarbon chain linked by a vinyl ether bond at the glycerol sn-1 position, and are involved in antioxidative and signaling mechanisms.
Protein Name Plasmanylethanolamine Desaturase 1
Transmembrane Protein 189
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Plasmanylethanolamine Desaturase 1 antibody
Anti-Transmembrane Protein 189 antibody
Anti-PEDS1 antibody
Anti-KUA antibody
Anti-PDES antibody
Anti-TMEM189 antibody

Information sourced from Uniprot.org

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