• Immunofluorescence analysis of paraffin-embedded mouse spleen using TLR8 Rabbit polyclonal antibody (STJ25872) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat spleen using TLR8 Rabbit polyclonal antibody (STJ25872) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of TLR8 in paraffin-embedded human lung cancer using TLR8 Rabbit polyclonal antibody (STJ25872) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TLR8 in paraffin-embedded human liver cancer using TLR8 Rabbit polyclonal antibody (STJ25872) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TLR8 in paraffin-embedded human colon using TLR8 Rabbit polyclonal antibody (STJ25872) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HepG2 cells, using TLR8 Rabbit polyclonal antibody (STJ25872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TLR8 antibody (849-1041) (STJ25872)

SKU:
STJ25872

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TLR8 (849-1041) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TLR8
Gene ID: 51311
Uniprot ID: TLR8_HUMAN
Immunogen Region: 849-1041
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 849-1041 of human TLR8 (NP_619542.1).
Immunogen Sequence: HHLFYWDVWFIYNVCLAKVK GYRSLSTSQTFYDAYISYDT KDASVTDWVINELRYHLEES RDKNVLLCLEERDWDPGLAI IDNLMQSINQSKKTVFVLTK KYAKSWNFKTAFYLALQRLM DENMDVIIFILLEPVLQHSQ YLRLRQRICKSSILQWPDNP KAEGLFWQTLRNVVLTENDS RYNNMYVDSIKQY
Tissue Specificity Expressed in myeloid dendritic cells, monocytes, and monocyte-derived dendritic cells.
Post Translational Modifications Ubiquitinated by RNF216.leading to degradation by the proteasome. Proteolytic processing occurs in monocytes and monocyte-derived macrophages by both furin-like proprotein convertase and cathepsins. The cleavage is necessary for dimer formation and subsequent activation.
Function Endosomal receptor that plays a key role in innate and adaptive immunity. Controls host immune response against pathogens through recognition of RNA degradation products specific to microorganisms that are initially processed by RNASET2. Recognizes GU-rich single-stranded RNA (GU-rich RNA) derived from SARS-CoV-2, SARS-CoV-1 and HIV-1 viruses. Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction. In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce pro-inflammatory cytokines and interferons, respectively.
Protein Name Toll-Like Receptor 8
Cd Antigen Cd288
Database Links Reactome: R-HSA-1679131
Reactome: R-HSA-168181
Reactome: R-HSA-9705671
Cellular Localisation Endosome Membrane
Single-Pass Type I Membrane Protein
Endosomal Localization Confers Distinctive Proteolytic Processing
Alternative Antibody Names Anti-Toll-Like Receptor 8 antibody
Anti-Cd Antigen Cd288 antibody
Anti-TLR8 antibody
Anti-UNQ249 antibody
Anti-PRO286 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance