• Western blot analysis of extracts of mouse liver, using TLR7 antibody (STJ25871) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of mouse spleen cells using TLR7 rabbit polyclonal antibody (STJ25871) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat spleen cells using TLR7 rabbit polyclonal antibody (STJ25871) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of THP-1 cells using TLR7 rabbit polyclonal antibody (STJ25871) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse spleen cells using TLR7 rabbit polyclonal antibody (STJ25871) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat spleen cells using TLR7 rabbit polyclonal antibody (STJ25871) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-TLR7 antibody (30-1049) (STJ25871)

SKU:
STJ25871

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TLR7 (30-1049) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TLR7
Gene ID: 51284
Uniprot ID: TLR7_HUMAN
Immunogen Region: 30-1049
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-1049 of human TLR7 (NP_057646.1).
Immunogen Sequence: FPKTLPCDVTLDVPKNHVIV DCTDKHLTEIPGGIPTNTTN LTLTINHIPDISPASFHRLD HLVEIDFRCNCVPIPLGSKN NMCIKRLQIKPRSFSGLTYL KSLYLDGNQLLEIPQGLPPS LQLLSLEANNIFSIRKENLT ELANIEILYLGQNCYYRNPC YVSYSIEKDAFLNLTKLKVL SLKDNNVTAVPTVLPSTLTE L
Tissue Specificity Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells. Expressed in peripheral mononuclear blood cells.
Function Endosomal receptor that plays a key role in innate and adaptive immunity. Controls host immune response against pathogens through recognition of uridine-containing single strand RNAs (ssRNAs) of viral origin or guanosine analogs. Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction. In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce pro-inflammatory cytokines and interferons, respectively.
Protein Name Toll-Like Receptor 7
Database Links Reactome: R-HSA-1679131
Reactome: R-HSA-168181
Reactome: R-HSA-9679191
Reactome: R-HSA-9692916
Reactome: R-HSA-9705671
Reactome: R-HSA-975110
Reactome: R-HSA-975138
Reactome: R-HSA-975155
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Endosome
Lysosome
Cytoplasmic Vesicle
Phagosome
Relocalizes From Endoplasmic Reticulum To Endosome And Lysosome Upon Stimulation With Agonist
Alternative Antibody Names Anti-Toll-Like Receptor 7 antibody
Anti-TLR7 antibody
Anti-UNQ248 antibody
Anti-PRO285 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance