Anti-TLR7 antibody (30-1049) (STJ25871)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TLR7 (30-1049) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TLR7 |
| Gene ID | 51284 |
| Uniprot ID | TLR7_HUMAN |
| Immunogen Region | 30-1049 |
| Immunogen Sequence | FPKTLPCDVTLDVPKNHVIV DCTDKHLTEIPGGIPTNTTN LTLTINHIPDISPASFHRLD HLVEIDFRCNCVPIPLGSKN NMCIKRLQIKPRSFSGLTYL KSLYLDGNQLLEIPQGLPPS LQLLSLEANNIFSIRKENLT ELANIEILYLGQNCYYRNPC YVSYSIEKDAFLNLTKLKVL SLKDNNVTAVPTVLPSTLTE L |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 30-230 of human TLR7 (NP_057646.1). |
Additional Info
| Tissue Specificity | Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells. Expressed in peripheral mononuclear blood cells. |
| Function | Endosomal receptor that plays a key role in innate and adaptive immunity. Controls host immune response against pathogens through recognition of uridine-containing single strand RNAs (ssRNAs) of viral origin or guanosine analogs. Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction. In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce pro-inflammatory cytokines and interferons, respectively. In plasmacytoid dendritic cells, RNASET2 endonuclease cooperates with PLD3 or PLD4 5'->3' exonucleases to process RNA and release 2',3'-cyclic guanosine monophosphate (2',3'-cGMP) and cytidine-rich RNA fragments that occupy TLR7 ligand-binding pockets and trigger a signaling-competent state. |
| Protein Name | Toll-Like Receptor 7 |
| Database Links | Reactome: R-HSA-1679131Reactome: R-HSA-168181Reactome: R-HSA-5686938Reactome: R-HSA-9679191Reactome: R-HSA-9692916Reactome: R-HSA-9705671Reactome: R-HSA-975110Reactome: R-HSA-975138Reactome: R-HSA-975155Reactome: R-HSA-9824856Reactome: R-HSA-9833110 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type I Membrane ProteinEndosomeLysosomeCytoplasmic VesiclePhagosomeRelocalizes From Endoplasmic Reticulum To Endosome And Lysosome Upon Stimulation With Agonist |
| Alternative Antibody Names | Anti-Toll-Like Receptor 7 antibodyAnti-TLR7 antibodyAnti-UNQ248 antibodyAnti-PRO285 antibody |
Information sourced from Uniprot.org