• Anti-ZO1 antibody at 1:2500 dilution; 50 µg of total protein per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Immunoperoxidase of polyclonal antibody to ZO1 (1:200) on paraformaldehyde-fixed paraffin-embedded mouse kidney
  • Immunofluorescence of primary RPE cells with ZO1 antibody at 1:100 dilution
  • Immunofluorescence of primary RPE cells with ZO1 antibody at 1:100 dilution
  • Immunofluorescence of primary RPE cells with ZO1 antibody at 1:100 dilution
  • Immunofluorescence of primary RPE cells with ZO1 antibody at 1:100 dilution
  • Immunofluorescence of primary RPE cells with ZO1 antibody at 1:100 dilution

Anti-TJP1 antibody (1580aa C-Term) (STJ140055)

SKU:
STJ140055

£100.50 - £393.50
Current Stock:
Host: Goat
Applications: WB/IHC-F/IHC-P/IF
Reactivity: Human/Rat/Mouse/Monkey/Canine
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Tight junction protein 1 (1580aa C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3 mg/ml
Dilution Range: WB 1:500-1:2000
IF 1:50-1:250
IHC-P 1:200-1:1000
IHC-F 1:200-1:1000
Cardoso MHS PhD Thesis NOVA University of Lisbon Portugal 2018
Pelkonen L Sato K Reinisalo M et al. Mol Pharm. 2017 Mar 6. Suplemental Material. PMID: 28112518
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Gene Symbol: TJP1
Gene ID: 7082
Uniprot ID: ZO1_HUMAN
Immunogen Region: 1580aa C-Term
Accession Number: ENSG00000104067
Specificity: Detects endogenous levels of total ZO1 by Western blot in whole cell and tissue lysates.
Immunogen: Purified recombinant peptide derived from within residues 1580 aa to the C-terminus of human ZO-1 produced in E. coli.
Immunogen Sequence: PQGAIPEGVEQEIYFKVCRD NSILPPLDKEKGETLLSPLV MCGPHGLKFLKPVELRLPHC DPKTWQNKCLPGDPNYLVGA NCVSVLIDHF
Tissue Specificity The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
Post Translational Modifications Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF). This response is dependent on an intact actin microfilament system. Dephosphorylated by PTPRJ.
Function TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling. With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus.
Protein Name Tight Junction Protein Zo-1
Tight Junction Protein 1
Zona Occludens Protein 1
Zonula Occludens Protein 1
Database Links Reactome: R-HSA-191650
Reactome: R-HSA-2028269
Reactome: R-HSA-351906
Reactome: R-HSA-8935964
Reactome: R-HSA-9705677
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Junction
Tight Junction
Gap Junction
Cell Projection
Podosome
Moves From The Cytoplasm To The Cell Membrane Concurrently With Cell-Cell Contact
At Podosomal Sites
Is Predominantly Localized In The Ring Structure Surrounding The Actin Core
Colocalizes With Spef1 At Sites Of Cell-Cell Contact In Intestinal Epithelial Cells
Alternative Antibody Names Anti-Tight Junction Protein Zo-1 antibody
Anti-Tight Junction Protein 1 antibody
Anti-Zona Occludens Protein 1 antibody
Anti-Zonula Occludens Protein 1 antibody
Anti-TJP1 antibody
Anti-ZO1 antibody

Information sourced from Uniprot.org

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