| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ZO-1 (18-1748) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TJP1 |
| Gene ID: |
7082 |
| Uniprot ID: |
ZO1_HUMAN |
| Immunogen Region: |
18-1748 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 18-1748 of human ZO-1 (NP_003248.3). |
| Immunogen Sequence: |
IWEQHTVTLHRAPGFGFGIA ISGGRDNPHFQSGETSIVIS DVLKGGPAEGQLQENDRVAM VNGVSMDNVEHAFAVQQLRK SGKNAKITIRRKKKVQIPVS RPDPEPVSDNEEDSYDEEIH DPRSGRSGVVNRRSEKIWPR DRSASRERSLSPRSDRRSVA SSQPAKPTKVTLVKSRKNEE YGLRLASHIFVKEISQDSLA ARDGNIQEGDVVLKINGTVT ENMSLTDAKTLIERSKGKL |
| Tissue Specificity | The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. |
| Post Translational Modifications | Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF). This response is dependent on an intact actin microfilament system. Dephosphorylated by PTPRJ. |
| Function | TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling. With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus. |
| Protein Name | Tight Junction Protein Zo-1Tight Junction Protein 1Zona Occludens Protein 1Zonula Occludens Protein 1 |
| Database Links | Reactome: R-HSA-191650Reactome: R-HSA-2028269Reactome: R-HSA-351906Reactome: R-HSA-8935964Reactome: R-HSA-9705677 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmic SideCell JunctionTight JunctionGap JunctionCell ProjectionPodosomeMoves From The Cytoplasm To The Cell Membrane Concurrently With Cell-Cell ContactAt Podosomal SitesIs Predominantly Localized In The Ring Structure Surrounding The Actin CoreColocalizes With Spef1 At Sites Of Cell-Cell Contact In Intestinal Epithelial Cells |
| Alternative Antibody Names | Anti-Tight Junction Protein Zo-1 antibodyAnti-Tight Junction Protein 1 antibodyAnti-Zona Occludens Protein 1 antibodyAnti-Zonula Occludens Protein 1 antibodyAnti-TJP1 antibodyAnti-ZO1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
