| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-TIRAP (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 22-23kDa band observed in lysates of cell lines A549 and K562 and in Human colorectal cancer lysates (calculated MW of 23.9kDa according to NP_001034750.11). Recommended concent |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | TIRAP |
| Gene ID: | 114609 |
| Uniprot ID: | TIRAP_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_001034750.1; NP_683708.1 |
| Specificity: | This antibody is expected to recognize both reported isoforms (NP_001034750.1 and NP_683708.1). |
| Immunogen Sequence: | QTLLKKPKKRPNSPE |
| Post Translational Modifications | Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK. Polyubiquitinated. Polyubiquitination follows phosphorylation by BTK and leads to TIRAP degradation. |
| Function | Adapter involved in TLR2, TLR4 and RAGE signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha (TNF) and interleukin-6 (IL6). |
| Protein Name | Toll/Interleukin-1 Receptor Domain-Containing Adapter ProteinTir Domain-Containing Adapter ProteinAdaptor Protein WyattMyd88 Adapter-Like ProteinMyd88-2 |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-166058Reactome: R-HSA-5602498Reactome: R-HSA-5603041 |
| Cellular Localisation | CytoplasmCell MembraneMembraneColocalizes With Dab2ip At The Plasma Membrane |
| Alternative Antibody Names | Anti-Toll/Interleukin-1 Receptor Domain-Containing Adapter Protein antibodyAnti-Tir Domain-Containing Adapter Protein antibodyAnti-Adaptor Protein Wyatt antibodyAnti-Myd88 Adapter-Like Protein antibodyAnti-Myd88-2 antibodyAnti-TIRAP antibodyAnti-MAL antibody |
Information sourced from Uniprot.org