• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using TIRAP antibody (STJ25855) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using TIRAP antibody (STJ25855) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-TIRAP antibody (1-221) (STJ25855)

SKU:
STJ25855

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Host: Rabbit
Applications: IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TIRAP (1-221) is suitable for use in Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TIRAP
Gene ID: 114609
Uniprot ID: TIRAP_HUMAN
Immunogen Region: 1-221
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human TIRAP (NP_001034750.1).
Immunogen Sequence: MASSTSLPAPGSRPKKPLGK MADWFRQTLLKKPKKRPNSP ESTSSDASQPTSQDSPLPPS LSSVTSPSLPPTHASDSGSS RWSKDYDVCVCHSEEDLVAA QDLVSYLEGSTASLRCFLQL RDATPGGAIVSELCQALSSS HCRVLLITPGFLQDPWCKYQ MLQALTEAPGAEGCTIPLLS GLSRAAYPPELRFMYYVDGR GPDGGFRQVKEAVMRYLQTL S
Tissue Specificity Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain.
Post Translational Modifications Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK. Polyubiquitinated. Polyubiquitination follows phosphorylation by BTK and leads to TIRAP degradation.
Function Adapter involved in TLR2, TLR4 and RAGE signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha (TNF) and interleukin-6 (IL6).
Protein Name Toll/Interleukin-1 Receptor Domain-Containing Adapter Protein
Tir Domain-Containing Adapter Protein
Adaptor Protein Wyatt
Myd88 Adapter-Like Protein
Myd88-2
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-166058
Reactome: R-HSA-5602498
Reactome: R-HSA-5603041
Cellular Localisation Cytoplasm
Cell Membrane
Membrane
Colocalizes With Dab2ip At The Plasma Membrane
Alternative Antibody Names Anti-Toll/Interleukin-1 Receptor Domain-Containing Adapter Protein antibody
Anti-Tir Domain-Containing Adapter Protein antibody
Anti-Adaptor Protein Wyatt antibody
Anti-Myd88 Adapter-Like Protein antibody
Anti-Myd88-2 antibody
Anti-TIRAP antibody
Anti-MAL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance