• Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-TIMP1 (ABT-TIMP1) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 23kDaObserved band size: 23kDa

Anti-TIMP1 antibody (50-150 aa) [ABT-TIMP1] (STJ197007)

SKU:
STJ197007

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/IF/WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Metalloproteinase inhibitor 1 (50-150 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and Western Blot research applications.
Clonality: Monoclonal
Clone ID: ABT-TIMP1
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:50-100
IF 1:50-100
WB 20-50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TIMP1
Gene ID: 7076
Uniprot ID: TIMP1_HUMAN
Immunogen Region: 50-150 aa
Specificity: This antibody detects endogenous levels of human Tissue Inhibitor of Metalloproteinases 1 (TIMP1). Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair
Immunogen: Synthesized peptide derived from the human Tissue Inhibitor of Metalloproteinases 1 (TIMP1) at the amino acid range 50-150
Post Translational Modifications The activity of TIMP1 is dependent on the presence of disulfide bonds. N-glycosylated.
Function Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro.but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.
Protein Name Metalloproteinase Inhibitor 1
Erythroid-Potentiating Activity
Epa
Fibroblast Collagenase Inhibitor
Collagenase Inhibitor
Tissue Inhibitor Of Metalloproteinases 1
Timp-1
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1592389
Reactome: R-HSA-381426
Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative Antibody Names Anti-Metalloproteinase Inhibitor 1 antibody
Anti-Erythroid-Potentiating Activity antibody
Anti-Epa antibody
Anti-Fibroblast Collagenase Inhibitor antibody
Anti-Collagenase Inhibitor antibody
Anti-Tissue Inhibitor Of Metalloproteinases 1 antibody
Anti-Timp-1 antibody
Anti-TIMP1 antibody
Anti-CLGI antibody
Anti-TIMP antibody

Information sourced from Uniprot.org

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