• Western blot analysis of various lysates, using TIMM22 Rabbit monoclonal antibody (STJ11105150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of HepG2, using TIMM22 Rabbit monoclonal antibody (STJ11105150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-TIMM22 antibody (20-120) [S5150RM] (STJ11105150)

SKU:
STJ11105150

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Mitochondrial Import Inner Membrane Translocase Subunit Tim22 (20-120) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5150RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TIMM22
Gene ID: 29928
Uniprot ID: TIM22_HUMAN
Immunogen Region: 20-120
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 20-120aa of human TIMM22.
Post Translational Modifications Disulfide bonds promote efficient assembly of the TIM22 complex.
Function Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps.
Protein Name Mitochondrial Import Inner Membrane Translocase Subunit Tim22
Testis-Expressed Protein 4
Database Links Reactome: R-HSA-1268020
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Mitochondrial Import Inner Membrane Translocase Subunit Tim22 antibody
Anti-Testis-Expressed Protein 4 antibody
Anti-TIMM22 antibody
Anti-TEX4 antibody
Anti-TIM22 antibody

Information sourced from Uniprot.org

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