• Western blot analysis of extracts of various cell lines, using TIMM10B antibody (STJ110786) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using TIMM10B antibody (STJ110786) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse stomach using TIMM10B antibody (STJ110786) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse stomach using TIMM10B antibody (STJ110786) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver cancer using TIMM10B antibody (STJ110786) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using TIMM10B antibody (STJ110786) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TIMM10B antibody (1-103) (STJ110786)

SKU:
STJ110786

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TIMM10B (1-103) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TIMM10B
Gene ID: 26515
Uniprot ID: T10B_HUMAN
Immunogen Region: 1-103
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-103 of human TIMM10B (NP_036324.1).
Immunogen Sequence: MERQQQQQQQLRNLRDFLLV YNRMTELCFQRCVPSLHHRA LDAEEEACLHSCAGKLIHSN HRLMAAYVQLMPALVQRRIA DYEAASAVPGVAAEQPGVSP SGS
Tissue Specificity Ubiquitous, with highest expression in heart, kidney, liver and skeletal muscle.
Function Component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. The TIM22 complex forms a twin-pore translocase that uses the membrane potential as the external driving force. In the TIM22 complex, it may act as a docking point for the soluble 70 kDa complex that guides the target proteins in transit through the aqueous mitochondrial intermembrane space.
Protein Name Mitochondrial Import Inner Membrane Translocase Subunit Tim10 B
Fracture Callus Protein 1
Fxc1
Mitochondrial Import Inner Membrane Translocase Subunit Tim9 B
Timm10b
Tim10b
Database Links Reactome: R-HSA-1268020
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Mitochondrial Import Inner Membrane Translocase Subunit Tim10 B antibody
Anti-Fracture Callus Protein 1 antibody
Anti-Fxc1 antibody
Anti-Mitochondrial Import Inner Membrane Translocase Subunit Tim9 B antibody
Anti-Timm10b antibody
Anti-Tim10b antibody
Anti-TIMM10B antibody
Anti-FXC1 antibody
Anti-TIM9B antibody
Anti-TIMM9B antibody

Information sourced from Uniprot.org

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