| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-TIM50 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 39kDa in Human Heart lysate (Calculated MW of 50.5kDa according to NP_001001563.1). The observed molecular weight corresponds to earlier finding in literature with different an |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | TIMM50 |
| Gene ID: | 92609 |
| Uniprot ID: | TIM50_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_001001563.1 |
| Specificity: | This antibody is expected to recognise an epitope corresponding to aa 396-409 of human TIM50. |
| Immunogen Sequence: | CDVRTVLEHYALEDD |
| Function | Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro.however such activity may not be relevant in vivo. Isoform 2: May participate in the release of snRNPs and SMN from the Cajal body. |
| Protein Name | Mitochondrial Import Inner Membrane Translocase Subunit Tim50 |
| Database Links | Reactome: R-HSA-1268020 |
| Cellular Localisation | Mitochondrion Inner MembraneSingle-Pass Membrane ProteinIsoform 2: Nucleus SpeckleNuclear And Enriched In Speckles With Snrnps |
| Alternative Antibody Names | Anti-Mitochondrial Import Inner Membrane Translocase Subunit Tim50 antibodyAnti-TIMM50 antibodyAnti-TIM50 antibodyAnti-PRO1512 antibody |
Information sourced from Uniprot.org