• Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using TIFY11B Rabbit polyclonal antibody (STJ11104275) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-TIFY11B antibody (170-269) (STJ11104275)

SKU:
STJ11104275

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: A.thaliana
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TIFY11B (170-269) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 170-269
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 170-269 of arabidopsis thaliana TIFY11B (NP_565043.1).
Immunogen Sequence: NNEDQETGQQHQVVERIARR ASLHRFFAKRKDRAVARAPY QVNQHGSHLPPKPEMVAPSI KSGQSSQHIATPPKPKAHNH MPMEVDKKEGQSSKNLELKL
Background JAZ6 transcript levels rise in response to a jasmonate stimulus and a GFP:JAZ6 fusion protein localizes to the nucleus. Application of jasmonate methyl ester to Arabidopsis roots reduces the levels of a JAZ6:GUS fusion protein, presumably by stimulating ubiquitin-proteasome-mediated degradation.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance