| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IHC |
| Reactivity: |
Human/Mouse/Dog/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-TIAL1 & TIA (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
WB-1-3µg/mlIHC-Recommended concentration, 3-5µg/mlELISA-antibody detection limit dilution 1:64000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
TIAL1 |
| Gene ID: |
7073 |
| Uniprot ID: |
TIAR_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_003243.1; NP_001029097.1 |
| Specificity: |
This antibody is expected to recognize both isoforms (NP_003243.1; NP_001029097.1). |
| Immunogen Sequence: |
SPEITTEDIKSAFAP |
| Post Translational Modifications | Phosphorylated by MAPK14 following DNA damage, releasing TIAR from GADD45A mRNA. |
| Function | RNA-binding protein involved in alternative pre-RNA splicing and in cytoplasmic stress granules formation. Shows a preference for uridine-rich RNAs. Activates splicing of alternative exons with weak 5' splice sites followed by a U-rich stretch on its own pre-mRNA and on TIA1 mRNA. Promotes the inclusion of TIA1 exon 5 to give rise to the long isoform (isoform a) of TIA1. Acts downstream of the stress-induced phosphorylation of EIF2S1/EIF2A to promote the recruitment of untranslated mRNAs to cytoplasmic stress granules (SG). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. |
| Protein Name | Nucleolysin TiarTia-1-Related Protein |
| Database Links | Reactome: R-HSA-6803529 |
| Cellular Localisation | NucleusCytoplasmCytolytic GranuleStress GranuleNuclear Import Seems To Be Coupled To Rna Polymerase Ii Transcription And May Be Dependent On Rna-BindingAccumulates In Cytoplasmic Stress Granules (Sg) Following Cellular Damage |
| Alternative Antibody Names | Anti-Nucleolysin Tiar antibodyAnti-Tia-1-Related Protein antibodyAnti-TIAL1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
