| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Nucleolysin TIAR is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TIAL1 |
| Gene ID: |
7073 |
| Uniprot ID: |
TIAR_HUMAN |
| Specificity: |
This antibody detects endogenous levels of TIAR at Human, Mouse |
| Immunogen: |
Synthesized peptide derived from human TIAR |
| Post Translational Modifications | Phosphorylated by MAPK14 following DNA damage, releasing TIAR from GADD45A mRNA. |
| Function | RNA-binding protein involved in alternative pre-RNA splicing and in cytoplasmic stress granules formation. Shows a preference for uridine-rich RNAs. Activates splicing of alternative exons with weak 5' splice sites followed by a U-rich stretch on its own pre-mRNA and on TIA1 mRNA. Promotes the inclusion of TIA1 exon 5 to give rise to the long isoform (isoform a) of TIA1. Acts downstream of the stress-induced phosphorylation of EIF2S1/EIF2A to promote the recruitment of untranslated mRNAs to cytoplasmic stress granules (SG). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. |
| Protein Name | Nucleolysin TiarTia-1-Related Protein |
| Database Links | Reactome: R-HSA-6803529 |
| Cellular Localisation | NucleusCytoplasmCytolytic GranuleStress GranuleNuclear Import Seems To Be Coupled To Rna Polymerase Ii Transcription And May Be Dependent On Rna-BindingAccumulates In Cytoplasmic Stress Granules (Sg) Following Cellular Damage |
| Alternative Antibody Names | Anti-Nucleolysin Tiar antibodyAnti-Tia-1-Related Protein antibodyAnti-TIAL1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
