• Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.
  • Immunofluorescence of human iPSC-derived dopaminergic neurons labeled with anti-tyrosine hydroxylase (STJA0003816, green, 1:500). The blue stain is DAPI. Before labeling, cells were fixed with 4% PFA and permeabilized with 0. 1 to 0. 3 % Triton X. This image is kindly provided by Aurelie de Rus Jacquet, Howard Hughes Medical Institute, Janelia Research Campus.
  • Immunostaining of E17 rat midbrain mixed neuronal cultures showing TH positive neurons (STJA0003816, green, 1:500) and MAP2 (STJA0003676, red, 1:2000). Before labeling, cells were fixed with 4% PFA in PBS and permeabilized for 1 hour 0. 3 % Triton X. Image courtesy of Aurélie de Rus Jacquet, laboratory of Dr. Jean-Christophe Rochet, Purdue University.

Anti-TH antibody (STJA0003816)

SKU:
STJA0003816-100

£442.50
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Host: Rabbit
Applications: WB/IHC/ICC
Reactivity: All Mammalian/Chicken
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Tyrosine Hydroxylase is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC 1:1000
ICC 1:500-1:1000
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Th
Gene ID: 25085
Uniprot ID: TY3H_RAT
Immunogen: SDS-denatured, native rat tyrosine hydroxylase purification from pheochromocytoma.
Post Translational Modifications Phosphorylated on Ser-19, Ser-31 and Ser-40 by several protein kinases with different site specificities. Phosphorylation at Ser-31 and Ser-40 leads to an increase of TH activity. Phosphorylation at Ser-40 activates the enzyme and also counteracts the feedback inhibition of TH by catecholamines. Phosphorylation of Ser-19 and Ser-31 triggers the proteasomal degradation of TH through the ubiquitin-proteasome pathway. Phosphorylation at Ser-31 facilitates transport of TH from the soma to the nerve terminals via the microtubule network. Phosphorylation at Ser-19 induces the high-affinity binding to the 14-3-3 protein YWHAG.this interaction may influence the phosphorylation and dephosphorylation of other sites. Ser-19 increases the phosphorylation at Ser-40 in a hierarchical manner, leading to increased activity.
Function Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa. In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan but with lower specificity. Positively regulates the regression of retinal hyaloid vessels during postnatal development.
Protein Name Tyrosine 3-Monooxygenase
Tyrosine 3-Hydroxylase
Th
Database Links Reactome: R-RNO-209905
Cellular Localisation Cytoplasm
Perinuclear Region
Nucleus
Cell Projection
Axon
Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle
When Phosphorylated At Ser-19 Shows A Nuclear Distribution And When Phsphorylated At Ser-31 As Well At Ser-40 Shows A Cytosolic Distribution
Expressed In Dopaminergic Axons And Axon Terminals
Alternative Antibody Names Anti-Tyrosine 3-Monooxygenase antibody
Anti-Tyrosine 3-Hydroxylase antibody
Anti-Th antibody
Anti-Th antibody

Information sourced from Uniprot.org

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