• Immunofluorescence analysis of human skin cells using TGM3 Rabbit polyclonal antibody (STJ11105361) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and 293T cells transfected with TGM3 using TGM3 Rabbit polyclonal antibody (STJ11105361) at 1:2000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-TGM3 antibody (291-480) (STJ11105361)

SKU:
STJ11105361

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Protein-Glutamine Gamma-Glutamyltransferase E (291-480) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TGM3
Gene ID: 7053
Uniprot ID: TGM3_HUMAN
Immunogen Region: 291-480
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 291-480 of human TGM3 (NP_003236.3).
Immunogen Sequence: SRVITNFNSAHDTDRNLSVD VYYDPMGNPLDKGSDSVWNF HVWNEGWFVRSDLGPSYGGW QVLDATPQERSQGVFQCGPA SVIGVREGDVQLNFDMPFIF AEVNADRITWLYDNTTGKQW KNSVNSHTIGRYISTKAVGS NARMDVTDKYKYPEGSDQER QVFQKALGKLKPNTPFAATS SMGLETEEQE
Post Translational Modifications Activated by proteolytic processing. In vitro activation is commonly achieved by cleavage with dispase, a neutral bacterial protease. Dispase cleavage site was proposed to lie between Ser-470 and Ser-471 or between Pro-465 and Phe-466. Physiological activation may be catalyzed by CTSL and, to a lesser extent, by CTSS, but not by CTSB, CTSD nor CTSV.
Function Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold. In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath.
Protein Name Protein-Glutamine Gamma-Glutamyltransferase E
Transglutaminase E
Tg(E
Tge
Tgase E
Transglutaminase-3
Tgase-3 Cleaved Into - Protein-Glutamine Gamma-Glutamyltransferase E 50 Kda Catalytic Chain - Protein-Glutamine Gamma-Glutamyltransferase E 27 Kda Non-Catalytic Chain
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Protein-Glutamine Gamma-Glutamyltransferase E antibody
Anti-Transglutaminase E antibody
Anti-Tg(E antibody
Anti-Tge antibody
Anti-Tgase E antibody
Anti-Transglutaminase-3 antibody
Anti-Tgase-3 Cleaved Into - Protein-Glutamine Gamma-Glutamyltransferase E 50 Kda Catalytic Chain - Protein-Glutamine Gamma-Glutamyltransferase E 27 Kda Non-Catalytic Chain antibody
Anti-TGM3 antibody

Information sourced from Uniprot.org

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