• Immunohistochemistry analysis of paraffin-embedded mouse kidney using TCP1 Rabbit polyclonal antibody (STJ115327) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using TCP1 Rabbit polyclonal antibody (STJ115327) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using TCP1 antibody (STJ115327) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-TCP1 antibody (307-556) (STJ115327)

SKU:
STJ115327

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TCP1 (307-556) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TCP1
Gene ID: 6950
Uniprot ID: TCPA_HUMAN
Immunogen Region: 307-556
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 307-556 of human TCP1 (NP_110379.2).
Immunogen Sequence: AVRRVLKRDLKRIAKASGAT ILSTLANLEGEETFEAAMLG QAEEVVQERICDDELILIKN TKARTSASIILRGANDFMCD EMERSLHDALCVVKRVLESK SVVPGGGAVEAALSIYLENY ATSMGSREQLAIAEFARSLL VIPNTLAVNAAQDSTDLVAK LRAFHNEAQVNPERKNLKWI GLDLSNGKPRDNKQAGVFEP TIVKVKSLKFATEAAITILR IDDLIKLHPESKDDKHGSY
Function Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin (Probable).
Protein Name T-Complex Protein 1 Subunit Alpha
Tcp-1-Alpha
Cct-Alpha
Database Links Reactome: R-HSA-389957
Reactome: R-HSA-389960
Reactome: R-HSA-390450
Reactome: R-HSA-390471
Reactome: R-HSA-5620922
Reactome: R-HSA-6814122
Reactome: R-HSA-8950505
Cellular Localisation Cytoplasm
Cytosol
Cytoskeleton
Microtubule Organizing Center
Centrosome
Alternative Antibody Names Anti-T-Complex Protein 1 Subunit Alpha antibody
Anti-Tcp-1-Alpha antibody
Anti-Cct-Alpha antibody
Anti-TCP1 antibody
Anti-CCT1 antibody
Anti-CCTA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance