Anti-TBP antibody (411-426 aa) [R04-6D1] (STJA0012610)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human TATA binding protein TBP
General Information
| Short Description | Rabbit monoclonal anti-TATA Box Binding Protein (411-426 aa) for use in WB, IHC-F, IHC-P, ICC, IF and IP in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P/ICC/IF/IP |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R04-6D1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | TBP |
| Gene ID | 6908 |
| Uniprot ID | TBP_HUMAN |
| Immunogen | A synthetic peptide of human TATA binding protein TBP |
| Immunogen Region | 411-426 aa |
Additional Info
| Tissue Specificity | Widely expressed, with levels highest in the testis and ovary. |
| Function | The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C. TBP forms the TFIID-A module together with TAF3 and TAF5. TBP is a general transcription factor that functions at the core of the TFIID complex. During assembly of the core PIC on the promoter, as part of TFIID, TBP binds to and also bends promoter DNA, irrespective of whether the promoter contains a TATA box. Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. |
| Protein Name | Tata-Box-Binding ProteinTata Sequence-Binding ProteinTata-Binding FactorTata-Box FactorTranscription Initiation Factor Tfiid Tbp Subunit |
| Database Links | Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-427359Reactome: R-HSA-427413Reactome: R-HSA-5250924Reactome: R-HSA-674695Reactome: R-HSA-6804756Reactome: R-HSA-6807505Reactome: R-HSA-73762Reactome: R-HSA-73772Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-73863Reactome: R-HSA-749476Reactome: R-HSA-75953Reactome: R-HSA-76042Reactome: R-HSA-76061Reactome: R-HSA-76066Reactome: R-HSA-76071Reactome: R-HSA-9018519 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Tata-Box-Binding Protein antibodyAnti-Tata Sequence-Binding Protein antibodyAnti-Tata-Binding Factor antibodyAnti-Tata-Box Factor antibodyAnti-Transcription Initiation Factor Tfiid Tbp Subunit antibodyAnti-TBP antibodyAnti-GTF2D1 antibodyAnti-TF2D antibodyAnti-TFIID antibody |
Information sourced from Uniprot.org