• Confocal imaging of paraffin-embedded Mouse brain tissue using Talin 2 Rabbit monoclonal antibody (STJ11103151, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Rat brain tissue using Talin 2 Rabbit monoclonal antibody (STJ11103151, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of Talin 2 in paraffin-embedded human kidney tissue using Talin 2 Rabbit monoclonal antibody (STJ11103151) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Talin 2 in paraffin-embedded human liver cancer tissue using Talin 2 Rabbit monoclonal antibody (STJ11103151) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Talin 2 in paraffin-embedded human liver tissue using Talin 2 Rabbit monoclonal antibody (STJ11103151) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Talin 2 in paraffin-embedded human colon carcinoma tissue using Talin 2 Rabbit monoclonal antibody (STJ11103151) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using Talin 2 Rabbit monoclonal antibody (STJ11103151) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Talin 2 antibody (350-450) [S1MR] (STJ11103151)

SKU:
STJ11103151

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
IHC-P:1:500-1:2000
IF/ICC:1:100-1:400
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TLN2
Gene ID: 83660
Uniprot ID: TLN2_HUMAN
Immunogen Region: 350-450
Specificity: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human Talin 2 (Q9Y4G6).
Immunogen Sequence: VLQEWPLTTVKRWAASPKSF TLDFGEYQESYYSVQTTEGE QISQLIAGYIDIILKKKQSK DRFGLEGDEESTMLEESVSP KKSTILQQQFNRTGKAEHGS V
Function As a major component of focal adhesion plaques that links integrin to the actin cytoskeleton, may play an important role in cell adhesion. Recruits PIP5K1C to focal adhesion plaques and strongly activates its kinase activity.
Protein Name Talin-2
Cellular Localisation Cytoplasm
Cell Junction
Focal Adhesion
Synapse
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoskeleton
Focal Adhesion Plaques And Synapses
Alternative Antibody Names Anti-Talin-2 antibody
Anti-TLN2 antibody
Anti-KIAA0320 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance