• Immunofluorescence analysis of paraffin-embedded mouse brain using Syntaxin Rabbit monoclonal antibody (STJ11103153) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat brain using Syntaxin Rabbit monoclonal antibody (STJ11103153) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Syntaxin in paraffin-embedded rat brain tissue using Syntaxin Rabbit monoclonal antibody (STJ11103153) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Syntaxin in paraffin-embedded mouse testis tissue using Syntaxin Rabbit monoclonal antibody (STJ11103153) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Syntaxin in paraffin-embedded human colon tissue using Syntaxin Rabbit monoclonal antibody (STJ11103153) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Syntaxin in paraffin-embedded human brain tissue using Syntaxin Rabbit monoclonal antibody (STJ11103153) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Syntaxin in paraffin-embedded mouse brain tissue using Syntaxin Rabbit monoclonal antibody (STJ11103153) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Syntaxin in paraffin-embedded human pancreas tissue using Syntaxin Rabbit monoclonal antibody (STJ11103153) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from Rat spinal cord, using Syntaxin Rabbit monoclonal antibody (STJ11103153) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using Syntaxin Rabbit monoclonal antibody (STJ11103153) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Syntaxin antibody (1-100) [S3MR] (STJ11103153)

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STJ11103153

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Syntaxin (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STX1B
Gene ID: 112755
Uniprot ID: STX1B_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Syntaxin (P61266).
Immunogen Sequence: MKDRTQELRSAKDSDDEEEV VHVDRDHFMDEFFEQVEEIR GCIEKLSEDVEQVKKQHSAI LAAPNPDEKTKQELEDLTAD IKKTANKVRSKLKAIEQSIE
Post Translational Modifications Phosphorylated by CK2.
Function Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
Protein Name Syntaxin-1b
Syntaxin-1b1
Syntaxin-1b2
Database Links Reactome: R-HSA-5250971
Reactome: R-HSA-5682910
Cellular Localisation Isoform 1: Membrane
Single-Pass Type Iv Membrane Protein
Isoform 2: Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle
Colocalizes With Lamin A/C And Numa In Interphasic Nuclei
And With Numa And Gamma-Tubulin In The Pericentrosomal Region Of The Mitotic Spindle In Dividing Cells
Alternative Antibody Names Anti-Syntaxin-1b antibody
Anti-Syntaxin-1b1 antibody
Anti-Syntaxin-1b2 antibody
Anti-STX1B antibody
Anti-STX1B1 antibody
Anti-STX1B2 antibody

Information sourced from Uniprot.org

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