Anti-SYNE3 antibody (750-935) [S4212RM] (STJ11104212)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11104212
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General Information

Short DescriptionRabbit monoclonal Nesprin-3 (750-935) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS4212RM
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolSYNE3
Gene ID161176
Uniprot IDSYNE3_HUMAN
Immunogen Region750-935
Immunogen SequenceLIRNWHLQRMEVDSGKKMVF TNNIPKSGFLINPMDPIPRH RRRANLLQEEEGSHEDFSQL LRNFGQWLQVENSKLVRIIA MRTSTAEDLRTRKSKLQELE ARVPEGQHLFENLLRLGPAR GTSDELEDLRYQWMLYKSKL KDSGHLLTQSSPGEPTGFQK TRRWRGLGSLFRRACCVALP LQLLLL
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 750-935 of human Nesprin3 (NP_689805.3).

Additional Info

Tissue Specificity Expressed in aortic endothelial cells (at protein level).
Post Translational Modifications The disulfid bond with SUN1 or SUN2 is required for stability of the respective LINC complex under tensile forces.
Function As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells.
Protein Name Nesprin-3
Kash Domain-Containing Protein 3
Kash3
Nuclear Envelope Spectrin Repeat Protein 3
Database Links
Cellular Localisation Nucleus Outer Membrane
Single-Pass Type Iv Membrane Protein
Nucleus Envelope
Rough Endoplasmic Reticulum
Alternative Antibody Names Anti-Nesprin-3 antibody
Anti-Kash Domain-Containing Protein 3 antibody
Anti-Kash3 antibody
Anti-Nuclear Envelope Spectrin Repeat Protein 3 antibody
Anti-SYNE3 antibody
Anti-C14orf139 antibody
Anti-C14orf49 antibody
Anti-LINC00341 antibody

Information sourced from Uniprot.org

Citations

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