| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Nesprin-3 (750-935) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S4212RM |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SYNE3 |
| Gene ID: |
161176 |
| Uniprot ID: |
SYNE3_HUMAN |
| Immunogen Region: |
750-935 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 750-935 of human Nesprin3 (NP_689805.3). |
| Immunogen Sequence: |
LIRNWHLQRMEVDSGKKMVF TNNIPKSGFLINPMDPIPRH RRRANLLQEEEGSHEDFSQL LRNFGQWLQVENSKLVRIIA MRTSTAEDLRTRKSKLQELE ARVPEGQHLFENLLRLGPAR GTSDELEDLRYQWMLYKSKL KDSGHLLTQSSPGEPTGFQK TRRWRGLGSLFRRACCVALP LQLLLL |
| Tissue Specificity | Expressed in aortic endothelial cells (at protein level). |
| Post Translational Modifications | The disulfid bond with SUN1 or SUN2 is required for stability of the respective LINC complex under tensile forces. |
| Function | As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells. |
| Protein Name | Nesprin-3Kash Domain-Containing Protein 3Kash3Nuclear Envelope Spectrin Repeat Protein 3 |
| Cellular Localisation | Nucleus Outer MembraneSingle-Pass Type Iv Membrane ProteinNucleus EnvelopeRough Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-Nesprin-3 antibodyAnti-Kash Domain-Containing Protein 3 antibodyAnti-Kash3 antibodyAnti-Nuclear Envelope Spectrin Repeat Protein 3 antibodyAnti-SYNE3 antibodyAnti-C14orf139 antibodyAnti-C14orf49 antibodyAnti-LINC00341 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and DSG2 knockout (KO) A-549 cells, using [KO Validated] DSG2 Rabbit polyclonal antibody (STJ11100872) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89806/358273/STJ11100872_1__45236.1713122617.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and DSG2 knockout (KO) A-549 cells, using [KO Validated] DSG2 Rabbit polyclonal antibody (STJ11100872) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")