• Immunohistochemistry analysis of paraffin-embedded human kidney cancer using SUMO4 Antibody (STJ25751) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using SUMO4 Antibody (STJ25751) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human appendicitis using SUMO4 Antibody (STJ25751) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SUMO4 antibody (STJ25751) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-SUMO4 antibody (40-95) (STJ25751)

SKU:
STJ25751

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SUMO4 (40-95) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IHC-P 1:100-1:300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SUMO4
Gene ID: 387082
Uniprot ID: SUMO4_HUMAN
Immunogen Region: 40-95
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 40-95 of human SUMO4 (Q6EEV6).
Immunogen Sequence: LSKLMKAYCEPRGLSMKQIR FRFGGQPISGTDKPAQLEME DEDTIDVFQQPTGGVY
Tissue Specificity Expressed mainly in adult and embryonic kidney. Expressed at various levels in immune tissues, with the highest expression in the lymph node and spleen.
Post Translational Modifications In contrast to SUMO1, SUMO2 and SUMO3, seems to be insensitive to sentrin-specific proteases due to the presence of Pro-90. This may impair processing to mature form and conjugation to substrates.
Function Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may modulate protein subcellular localization, stability or activity. Upon oxidative stress, conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. May also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I.
Protein Name Small Ubiquitin-Related Modifier 4
Sumo-4
Small Ubiquitin-Like Protein 4
Alternative Antibody Names Anti-Small Ubiquitin-Related Modifier 4 antibody
Anti-Sumo-4 antibody
Anti-Small Ubiquitin-Like Protein 4 antibody
Anti-SUMO4 antibody
Anti-SMT3H4 antibody

Information sourced from Uniprot.org

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