| Tissue Specificity | Mainly expressed in liver, kidney, heart, spleen and skeletal muscle. Also found in intestine and colon, and in low amounts in lung, brain, prostate, testis and ovary. |
| Function | GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit. |
| Protein Name | Succinate--Coa Ligase Gdp-Forming Subunit Beta - MitochondrialGtp-Specific Succinyl-Coa Synthetase Subunit BetaG-ScsGtpscsSuccinyl-Coa Synthetase Beta-G ChainScs-Betag |
| Database Links | Reactome: R-HSA-71403Reactome: R-HSA-9837999 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Succinate--Coa Ligase Gdp-Forming Subunit Beta - Mitochondrial antibodyAnti-Gtp-Specific Succinyl-Coa Synthetase Subunit Beta antibodyAnti-G-Scs antibodyAnti-Gtpscs antibodyAnti-Succinyl-Coa Synthetase Beta-G Chain antibodyAnti-Scs-Betag antibodyAnti-SUCLG2 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and SUCLG2 knockout (KO) 293T (KO) cells, using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95318/410028/STJ116335_1__72895.1744896481.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SUCLG2 knockout (KO) 293T (KO) cells, using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
![Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95318/410029/STJ116335_2__92098.1744896482.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of paraffin-embedded Human lung cancer using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95318/410030/STJ116335_3__56183.1744896482.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded Human lung cancer using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.")
![Immunofluorescence analysis of C6 cells using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95318/410031/STJ116335_4__47807.1744896483.jpg?c=1 "Immunofluorescence analysis of C6 cells using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of U-2 OS cells using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95318/410032/STJ116335_5__95651.1744896483.jpg?c=1 "Immunofluorescence analysis of U-2 OS cells using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from wild type (WT) and SUCLG2 knockout (KO) 293T (KO) cells, using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/95318/410028/STJ116335_1__72895.1744896481.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SUCLG2 knockout (KO) 293T (KO) cells, using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
