Home
Primary Antibodies
Polyclonal Antibodies
Anti-SUCLG2 antibody (50-300) (STJ116335)

Western blot analysis of lysates from wild type (WT) and SUCLG2 knockout (KO) 293T (KO) cells, using [KO Validated] SUCLG2 Rabbit polyclonal antibody (STJ116335) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Western blot analysis of extracts of various cell lines, using SUCLG2 antibody (STJ116335) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Western blot analysis of lysates from wild type (WT) and SUCLG2 knockout (KO) 293T (KO) cells, using [KO Validated] SUCLG2 Rabbit polyclonal antibody (STJ116335) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Western blot analysis of lysates from wild type (WT) and SUCLG2 knockout (KO) 293T (KO) cells, using [KO Validated] SUCLG2 Rabbit polyclonal antibody (STJ116335) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SUCLG2 antibody (50-300) (STJ116335)

SKU:

STJ116335

£129.50 - £642.50

Size: Required 20 µL £129.50 100 µL £332.50 200 µL (2 x 100 µL) £642.50

Shipping:

Free Shipping

Current Stock:

General Information
Product Properties
Target Information
Additional Info

Host:	Rabbit
Applications:	WB/IHC-P/IF/ICC/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification:	Affinity purification
Concentration:	Lot specific
Dilution Range:	WB:1:1000-1:5000 IHC-P:1:50-1:200 IF/ICC:1:50-1:100 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	SUCLG2
Gene ID:	8801
Uniprot ID:	SUCB2_HUMAN
Immunogen Region:	50-300
Specificity:	Recombinant fusion protein containing a sequence corresponding to amino acids 50-300 of human SUCLG2 (NP_003839.2).
Immunogen Sequence:	SDNGVRVQRFFVADTANEAL EAAKRLNAKEIVLKAQILAG GRGKGVFNSGLKGGVHLTKD PNVVGQLAKQMIGYNLATKQ TPKEGVKVNKVMVAEALDIS RETYLAILMDRSCNGPVLVG SPQGGVDIEEVAASNPELIF KEQIDIFEGIKDSQAQRMAE NLGFVGPLKSQAADQITKLY NLFLKIDATQVEVNPFGETP EGQVVCFDAKINFDDNAEFR QKDIFAMDDKSENEPIENE

Tissue Specificity	Mainly expressed in liver, kidney, heart, spleen and skeletal muscle. Also found in intestine and colon, and in low amounts in lung, brain, prostate, testis and ovary.
Function	GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
Protein Name	Succinate--Coa Ligase Gdp-Forming Subunit Beta - Mitochondrial Gtp-Specific Succinyl-Coa Synthetase Subunit Beta G-Scs Gtpscs Succinyl-Coa Synthetase Beta-G Chain Scs-Betag
Database Links	Reactome: R-HSA-71403 Reactome: R-HSA-9837999
Cellular Localisation	Mitochondrion
Alternative Antibody Names	Anti-Succinate--Coa Ligase Gdp-Forming Subunit Beta - Mitochondrial antibody Anti-Gtp-Specific Succinyl-Coa Synthetase Subunit Beta antibody Anti-G-Scs antibody Anti-Gtpscs antibody Anti-Succinyl-Coa Synthetase Beta-G Chain antibody Anti-Scs-Betag antibody Anti-SUCLG2 antibody

Information sourced from Uniprot.org

reviews