• Immunohistochemistry analysis of paraffin-embedded mouse kidney using Syntaxin 3 Rabbit monoclonal antibody (STJ11101937) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using Syntaxin 3 Rabbit monoclonal antibody (STJ11101937) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Syntaxin 3 Rabbit monoclonal antibody (STJ11101937) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-STX3 antibody (100-200) [S7MR] (STJ11101937)

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STJ11101937

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Syntaxin 3 (100-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STX3
Gene ID: 6809
Uniprot ID: STX3_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Syntaxin 3 (Q13277).
Immunogen Sequence: KHIEEDEVRSSADLRIRKSQ HSVLSRKFVEVMTKYNEAQV DFRERSKGRIQRQLEITGKK TTDEELEEMLESGNPAIFTS GIIDSQISKQALSEIEGRHK D
Tissue Specificity Isoform A: Expressed in small intestine, kidney, pancreas, placenta as well as in retina. Weaker expression in lung, liver and heart. Not expressed in brain and skeletal muscle. Isoform B: Expressed only in the retina. Isoform 3: Ubiquitously expressed.
Function Potentially involved in docking of synaptic vesicles at presynaptic active zones. Apical receptor involved in membrane fusion of apical vesicles. Isoform B: Essential for survival of retinal photoreceetors. Isoform 3: Functions as a regulator of gene expression.
Protein Name Syntaxin-3
Database Links Reactome: R-HSA-449836
Cellular Localisation Isoform A: Apical Cell Membrane
Single-Pass Type Iv Membrane Protein
Localized To The Inner And Outer Plexiform Layers
The Cell Body And The Inner Segments Of Photoreceptors
Isoform 3: Nucleus
Alternative Antibody Names Anti-Syntaxin-3 antibody
Anti-STX3 antibody
Anti-STX3A antibody

Information sourced from Uniprot.org

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